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Open AccessArticle

Beiging Modulates Inflammatory Adipogenesis in Salt-Treated and MEK6–Transfected Adipocytes

by 1 and 1,2,*
1
Department of Food & Nutrition, Sungshin Women’s University, Seoul 01133, Korea
2
Research Institute of Obesity Sciences, Sungshin Women’s University, Seoul 01133, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Bruce A. Bunnell
Cells 2021, 10(5), 1106; https://doi.org/10.3390/cells10051106
Received: 26 March 2021 / Revised: 26 April 2021 / Accepted: 28 April 2021 / Published: 4 May 2021
(This article belongs to the Special Issue Research on Adipose Stem Cells)
To investigate whether the beiging process changes the interactive effects of salt and MEK6 gene on inflammatory adipogenesis, the salt treatment (NaCl 50 mM) and MEK6 transfection of Tg(+/+) cells were performed with white adipocytes (WAT) and beige-like-adipocytes (BLA). BLA induced by T3 were confirmed by UCP-1 expression and the MEK6 protein was 3.5 times higher in MEK6 transfected WAT than the control. The adipogenic genes, PPAR-γ and C/EBP-α, were 1.5 times more highly expressed in the salt-treated groups than the non-salt-treated groups, and adipogenesis was greatly increased in Tg(+/+) WAT compared to non-transfected Tg(−/−). The adipogenesis induced by salt treatment and MEK6 transfection was significantly reduced in BLA. The inflammatory adipocytokines, TNF-α, IL-1β, and IL-6, were increased in the salt-treated Tg(+/+) WAT, but an anti-inflammation biomarker, the adiponectin/leptin ratio, was reduced in Tg(+/+), to tenth of that in Tg(−/−). However, the production of adipocytokines in WAT was strongly weakened in BLA, although a combination of salt and MEK6 transfection had the most significant effects on inflammation in both WAT and BLA. Oxygen consumption in mitochondria was maximized in salt-treated and MEK6 transfected WAT, but it was decreased by 50% in BLA. In conclusion, beiging controls the synergistic effects of salt and MEK6 on adipogenesis, inflammation, and energy expenditure. View Full-Text
Keywords: obesity; beiging; beige-like adipocyte (BLA); white adipocyte (WAT); MEK6; salt obesity; beiging; beige-like adipocyte (BLA); white adipocyte (WAT); MEK6; salt
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MDPI and ACS Style

Kang, S.; Lee, M. Beiging Modulates Inflammatory Adipogenesis in Salt-Treated and MEK6–Transfected Adipocytes. Cells 2021, 10, 1106. https://doi.org/10.3390/cells10051106

AMA Style

Kang S, Lee M. Beiging Modulates Inflammatory Adipogenesis in Salt-Treated and MEK6–Transfected Adipocytes. Cells. 2021; 10(5):1106. https://doi.org/10.3390/cells10051106

Chicago/Turabian Style

Kang, Songjoo; Lee, Myoungsook. 2021. "Beiging Modulates Inflammatory Adipogenesis in Salt-Treated and MEK6–Transfected Adipocytes" Cells 10, no. 5: 1106. https://doi.org/10.3390/cells10051106

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