Alterations in the Gut-Microbial-Inflammasome-Brain Axis in a Mouse Model of Alzheimer’s Disease
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice and Brain Tissues
2.2. Microbiome Analysis
2.3. Relative Quantitative Real-Time Reverse-Transcriptase PCR (RT-qPCR)
2.4. Immunofluorescence Staining
2.5. ELISA
2.6. Western Blot Analyses
2.7. Statistical Analysis
3. Results
3.1. Altered Microbiome Composition in the Gut of Young and Old 5xFAD Mice
3.2. Compromised Gut Barrier Function in 5xFAD Mice
3.3. Increased NLRP3 Inflammasome and ASC Activation in the Gut of 5xFAD Mice
3.4. Caspase-1-Induced Inflammation in the Gut of 5xFAD Mice
3.5. Increased NLRP3 Inflammasome is Associated with Neuroinflammation in the Brains of 5xFAD Mice
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Shukla, P.K.; Delotterie, D.F.; Xiao, J.; Pierre, J.F.; Rao, R.; McDonald, M.P.; Khan, M.M. Alterations in the Gut-Microbial-Inflammasome-Brain Axis in a Mouse Model of Alzheimer’s Disease. Cells 2021, 10, 779. https://doi.org/10.3390/cells10040779
Shukla PK, Delotterie DF, Xiao J, Pierre JF, Rao R, McDonald MP, Khan MM. Alterations in the Gut-Microbial-Inflammasome-Brain Axis in a Mouse Model of Alzheimer’s Disease. Cells. 2021; 10(4):779. https://doi.org/10.3390/cells10040779
Chicago/Turabian StyleShukla, Pradeep K., David F. Delotterie, Jianfeng Xiao, Joseph F. Pierre, RadhaKrishna Rao, Michael P. McDonald, and Mohammad Moshahid Khan. 2021. "Alterations in the Gut-Microbial-Inflammasome-Brain Axis in a Mouse Model of Alzheimer’s Disease" Cells 10, no. 4: 779. https://doi.org/10.3390/cells10040779
APA StyleShukla, P. K., Delotterie, D. F., Xiao, J., Pierre, J. F., Rao, R., McDonald, M. P., & Khan, M. M. (2021). Alterations in the Gut-Microbial-Inflammasome-Brain Axis in a Mouse Model of Alzheimer’s Disease. Cells, 10(4), 779. https://doi.org/10.3390/cells10040779