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Open AccessArticle

Screening of EMS-Induced Drought-Tolerant Sugarcane Mutants Employing Physiological, Molecular and Enzymatic Approaches

by 1,2,3, 1,2,3, 2,4 and 1,2,3,4,*
1
National Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
2
College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
3
GMOs LAB of Quality Supervision Inspection & Testing Center for Sugarcane and Derived Products, Ministry of Agriculture, Fuzhou 350002, Fujian, China
4
Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
*
Author to whom correspondence should be addressed.
Agronomy 2018, 8(10), 226; https://doi.org/10.3390/agronomy8100226
Received: 3 September 2018 / Revised: 7 October 2018 / Accepted: 11 October 2018 / Published: 15 October 2018
Drought stress is one of the major agronomic concerns that lead towards a sharp decline in sugarcane yield. An urgent demand to overcome drought is critical to ensure sugarcane production. Mutation breeding is one of the promising tools available to produce stress-resistant plants, with the induction of new alleles due to point mutation within existing sugarcane germplasm. The current study was directed to chemically mutagenize the calli of two sugarcane cultivars (ROC22 and FN39) via 0.1% EMS, with focus on inducing mutations in their genome. The 1644 regenerated plants of ROC22 and 1398 of FN39 were exposed to 28% PEG-6000 stimulated osmotic stress. Eighteen plants of ROC22 and 2 plants of FN39, that survived after in vitro osmotic stress treatment, were then subjected to preliminary greenhouse pot trials to confirm drought tolerance by analyzing them using various physiological parameters, including photosystem II (PSII) photochemical efficiency (Fv/Fm), leaf chlorophyll content, and photosynthetic rate. The genetic diversity among drought-resistant mutant lines was further assessed by 15 pairs of simple sequence repeat (SSR) markers amplification and CEL (Celery) I endonuclease digestion, to investigate the mutated sites. Mutant lines of ROC22 (i.e., MR22-15 and MR22-20) were found to be promising for future drought resistance breeding, due to better physiological adaptation under drought stress. View Full-Text
Keywords: drought; mutation breeding; PSII photochemical efficiency; leaf chlorophyll content; photosynthetic rate; SSR marker analysis; CEL-I endonuclease digestion drought; mutation breeding; PSII photochemical efficiency; leaf chlorophyll content; photosynthetic rate; SSR marker analysis; CEL-I endonuclease digestion
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MDPI and ACS Style

Khalil, F.; Naiyan, X.; Tayyab, M.; Pinghua, C. Screening of EMS-Induced Drought-Tolerant Sugarcane Mutants Employing Physiological, Molecular and Enzymatic Approaches. Agronomy 2018, 8, 226. https://doi.org/10.3390/agronomy8100226

AMA Style

Khalil F, Naiyan X, Tayyab M, Pinghua C. Screening of EMS-Induced Drought-Tolerant Sugarcane Mutants Employing Physiological, Molecular and Enzymatic Approaches. Agronomy. 2018; 8(10):226. https://doi.org/10.3390/agronomy8100226

Chicago/Turabian Style

Khalil, Farghama; Naiyan, Xiao; Tayyab, Muhammad; Pinghua, Chen. 2018. "Screening of EMS-Induced Drought-Tolerant Sugarcane Mutants Employing Physiological, Molecular and Enzymatic Approaches" Agronomy 8, no. 10: 226. https://doi.org/10.3390/agronomy8100226

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