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Open AccessArticle

In Vitro Investigation of Thiol-Functionalized Cellulose Nanofibrils as a Chronic Wound Environment Modulator

1
Nanotechnology and Functional Materials, Department of Material Science and Engineering, Uppsala University, Box 35, 75103 Uppsala, Sweden
2
Department of Molecular Sciences, Swedish University of Agricultural Sciences, Box 7015, 75007 Uppsala, Sweden
*
Author to whom correspondence should be addressed.
Polymers 2021, 13(2), 249; https://doi.org/10.3390/polym13020249
Received: 8 December 2020 / Revised: 9 January 2021 / Accepted: 10 January 2021 / Published: 13 January 2021
(This article belongs to the Special Issue Biopolymers for Medical and Pharmaceutical Applications)
There is currently a huge need for new, improved therapeutic approaches for the treatment of chronic wounds. One promising strategy is to develop wound dressings capable of modulating the chronic wound environment (e.g., by controlling the high levels of reactive oxygen species (ROS) and proteases). Here, we selected the thiol-containing amino acid cysteine to endow wood-derived cellulose nanofibrils (CNF) with bioactivity toward the modulation of ROS levels and protease activity. Cysteine was covalently incorporated into CNF and the functionalized material, herein referred as cys-CNF, was characterized in terms of chemical structure, degree of substitution, radical scavenging capacity, and inhibition of protease activity. The stability of the thiol groups was evaluated over time, and an in vitro cytotoxicity study with human dermal fibroblasts was performed to evaluate the safety profile of cys-CNF. Results showed that cys-CNF was able to efficiently control the activity of the metalloprotease collagenase and to inhibit the free radical DPPH (1,1-Diphenyl-2-picrylhydrazyl radical), activities that were correlated with the presence of free thiol groups on the nanofibers. The stability study showed that the reactivity of the thiol groups challenged the bioactivity over time. Nevertheless, preparing the material as an aerogel and storing it in an inert atmosphere were shown to be valid approaches to increase the stability of the thiol groups in cys-CNF. No signs of toxicity were observed on the dermal fibroblasts when exposed to cys-CNF (concentration range 0.1–0.5 mg/mL). The present work highlights cys-CNF as a promising novel material for the development of bioactive wound dressings for the treatment of chronic wounds. View Full-Text
Keywords: nanocellulose; wound healing; proteases; reactive oxygen species; cysteine; antioxidant properties nanocellulose; wound healing; proteases; reactive oxygen species; cysteine; antioxidant properties
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MDPI and ACS Style

Blasi-Romero, A.; Palo-Nieto, C.; Sandström, C.; Lindh, J.; Strømme, M.; Ferraz, N. In Vitro Investigation of Thiol-Functionalized Cellulose Nanofibrils as a Chronic Wound Environment Modulator. Polymers 2021, 13, 249. https://doi.org/10.3390/polym13020249

AMA Style

Blasi-Romero A, Palo-Nieto C, Sandström C, Lindh J, Strømme M, Ferraz N. In Vitro Investigation of Thiol-Functionalized Cellulose Nanofibrils as a Chronic Wound Environment Modulator. Polymers. 2021; 13(2):249. https://doi.org/10.3390/polym13020249

Chicago/Turabian Style

Blasi-Romero, Anna; Palo-Nieto, Carlos; Sandström, Corine; Lindh, Jonas; Strømme, Maria; Ferraz, Natalia. 2021. "In Vitro Investigation of Thiol-Functionalized Cellulose Nanofibrils as a Chronic Wound Environment Modulator" Polymers 13, no. 2: 249. https://doi.org/10.3390/polym13020249

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