High Temperature Drives Topoisomerase Mediated Chromosomal Break Repair Pathway Choice
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. Hyperthermia Reduces the Catalytic Activity of TDP1 and TDP2
2.2. Hyperthermia Protects from Topoisomerase-Induced DNA Damage
2.3. Hyperthermia Favors an Error-Free Repair for Topoisomerase-Induced DNA Damage
2.4. Hyperthermia Reduces Topoisomerase-Induced Genomic Instability and Inflammation
3. Discussion
4. Methodology
4.1. Cell Culture, Synchronization, and Hyperthermia Treatment
4.2. Comet Assays
4.3. Cell Lysate Preparation
4.4. TDPs Activity Assay
4.5. Western Blot
4.6. Viability
4.7. TOP1 Activity Assay
4.8. Quantitative PCR
4.9. Chromosomal Translocation Assay
4.10. Micronuclei
4.11. Immunoprecipitation and Nano-LC MS/MS Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ashour, M.E.; Allam, W.; Elsayed, W.; Atteya, R.; Elserafy, M.; Magdeldin, S.; Hassan, M.K.; El-Khamisy, S.F. High Temperature Drives Topoisomerase Mediated Chromosomal Break Repair Pathway Choice. Cancers 2021, 13, 2315. https://doi.org/10.3390/cancers13102315
Ashour ME, Allam W, Elsayed W, Atteya R, Elserafy M, Magdeldin S, Hassan MK, El-Khamisy SF. High Temperature Drives Topoisomerase Mediated Chromosomal Break Repair Pathway Choice. Cancers. 2021; 13(10):2315. https://doi.org/10.3390/cancers13102315
Chicago/Turabian StyleAshour, Mohamed E., Walaa Allam, Waheba Elsayed, Reham Atteya, Menattallah Elserafy, Sameh Magdeldin, Mohamed K. Hassan, and Sherif F. El-Khamisy. 2021. "High Temperature Drives Topoisomerase Mediated Chromosomal Break Repair Pathway Choice" Cancers 13, no. 10: 2315. https://doi.org/10.3390/cancers13102315