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Ultrahigh-Throughput Improvement and Discovery of Enzymes Using Droplet-Based Microfluidic Screening

Université de Strasbourg, CNRS, Architecture et Réactivité de l’ARN, UPR 9002, F-67000 Strasbourg, France
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Academic Editors: Andrew J. deMello and Xavier Casadevall i Solvas
Micromachines 2017, 8(4), 128; https://doi.org/10.3390/mi8040128
Received: 21 January 2017 / Revised: 11 April 2017 / Accepted: 13 April 2017 / Published: 18 April 2017
(This article belongs to the Special Issue Droplet Microfluidics: Techniques and Technologies, Volume II)
Enzymes are extremely valuable tools for industrial, environmental, and biotechnological applications and there is a constant need for improving existing biological catalysts and for discovering new ones. Screening microbe or gene libraries is an efficient way of identifying new enzymes. In this view, droplet-based microfluidics appears to be one of the most powerful approaches as it allows inexpensive screenings in well-controlled conditions and an ultrahigh-throughput regime. This review aims to introduce the main microfluidic devices and concepts to be considered for such screening before presenting and discussing the latest successful applications of the technology for enzyme discovery. View Full-Text
Keywords: droplet-based microfluidics; high-throughput screening; enzyme improvement; directed evolution; single-cell droplet-based microfluidics; high-throughput screening; enzyme improvement; directed evolution; single-cell
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Autour, A.; Ryckelynck, M. Ultrahigh-Throughput Improvement and Discovery of Enzymes Using Droplet-Based Microfluidic Screening. Micromachines 2017, 8, 128.

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