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Open AccessArticle

Height Resolution of Antibody Spots Measured by Spinning-Disk Interferometry on the BioCD

1
Department of Physics, University of California Berkeley, Berkeley, CA 94720-7300, USA
2
Department of Physics, Purdue University, West Lafayette, IN 47907, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Marc Madou and Nam-Trung Nguyen
Micromachines 2016, 7(2), 31; https://doi.org/10.3390/mi7020031
Received: 3 December 2015 / Revised: 20 January 2016 / Accepted: 4 February 2016 / Published: 17 February 2016
(This article belongs to the Special Issue Centrifugal (Compact-Disc) Microfluidics for Extreme POC)
Spinning-disc interferometry (SDI) is a high-speed laser scanning approach to surface metrology that uses common-path interferometry to measure protein spots on a BioCD disk. The measurement sensitivity depends on the scanning pitch and on the time-base. Based on high-resolution laser scanning images of printed antibody spots, we quantify the protein sensitivity as a function of the scan parameters. For smoothly printed antibody spots scanned with a transverse spatial resolution of 1 μm, the surface height precision for a single 100 μm diameter protein spot is approximately 1 pm. This detection sensitivity sets the fundamental limit of detection for label-free BioCD biosensors performing immunoassays. View Full-Text
Keywords: differential phase contrast; common-path interferometry; silicon metrology; molecular interferometry; protein immobilization differential phase contrast; common-path interferometry; silicon metrology; molecular interferometry; protein immobilization
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MDPI and ACS Style

O’Brien, K.; Zhao, M.; Nolte, D. Height Resolution of Antibody Spots Measured by Spinning-Disk Interferometry on the BioCD. Micromachines 2016, 7, 31.

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