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Fabrication of Stromal Cell-Derived Factor-1 Contained in Gelatin/Hyaluronate Copo006Cymer Mixed with Hydroxyapatite for Use in Traumatic Bone Defects

1
Department of Biomedical Engineering, National Taiwan University, No. 1, Sec. 1, Jen-Ai Road, Taipei City 10051, Taiwan
2
Department of Orthopaedic Surgery, National Taiwan University Hospital, No. 7, Chung Shan South Road, Taipei City 10002, Taiwan
3
Integrative Stem Cell Center, China Medical University, No. 2, Yude Road, Taichung City 40447, Taiwan
*
Authors to whom correspondence should be addressed.
Academic Editors: Nur Mustafaoglu, Vasif Hasirci, Ken Takahashi and Menekse Ermis
Micromachines 2021, 12(7), 822; https://doi.org/10.3390/mi12070822
Received: 21 June 2021 / Revised: 8 July 2021 / Accepted: 13 July 2021 / Published: 14 July 2021
(This article belongs to the Special Issue 3D In Vitro Tissue and Organ Models)
Bone defects of orthopedic trauma remain a challenge in clinical practice. Regarding bone void fillers, besides the well-known osteoconductivity of most bone substitutes, osteoinductivity has also been gaining attention in recent years. It is known that stromal cell-derived factor-1 (SDF-1) can recruit mesenchymal stem cells (MSCs) in certain circumstances, which may also play an important role in bone regeneration. In this study, we fabricated a gelatin/hyaluronate (Gel/HA) copolymer mixed with hydroxyapatite (HAP) and SDF-1 to try and enhance bone regeneration in a bone defect model. After material characterization, these Gel/HA–HAP and Gel/HA–HAP–SDF-1 composites were tested for their biocompatibility and ability to recruit MSCs in vitro. A femoral condyle bone defect model of rats was used for in vivo studies. For the assessment of bone healing, micro-CT analysis, second harmonic generation (SHG) imaging, and histology studies were performed. As a result, the Gel/HA–HAP composites showed no systemic toxicity to rats. Gel/HA–HAP composite groups both showed better bone generation compared with the control group in an animal study, and the composite with the SDF-1 group even showed a trend of faster bone growth compared with the composite without SDF-1 group. In conclusion, in the management of traumatic bone defects, Gel/HA–HAP–SDF-1 composites can be a feasible material for use as bone void fillers. View Full-Text
Keywords: tissue engineering; biomaterial; hyaluronate; mesenchymal stem cells; hydroxyapatite; bone regeneration; gelatin; bone defect tissue engineering; biomaterial; hyaluronate; mesenchymal stem cells; hydroxyapatite; bone regeneration; gelatin; bone defect
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MDPI and ACS Style

Chang, Y.-L.; Hsieh, C.-Y.; Yeh, C.-Y.; Chang, C.-H.; Lin, F.-H. Fabrication of Stromal Cell-Derived Factor-1 Contained in Gelatin/Hyaluronate Copo006Cymer Mixed with Hydroxyapatite for Use in Traumatic Bone Defects. Micromachines 2021, 12, 822. https://doi.org/10.3390/mi12070822

AMA Style

Chang Y-L, Hsieh C-Y, Yeh C-Y, Chang C-H, Lin F-H. Fabrication of Stromal Cell-Derived Factor-1 Contained in Gelatin/Hyaluronate Copo006Cymer Mixed with Hydroxyapatite for Use in Traumatic Bone Defects. Micromachines. 2021; 12(7):822. https://doi.org/10.3390/mi12070822

Chicago/Turabian Style

Chang, Yun-Liang, Chia-Ying Hsieh, Chao-Yuan Yeh, Chih-Hao Chang, and Feng-Huei Lin. 2021. "Fabrication of Stromal Cell-Derived Factor-1 Contained in Gelatin/Hyaluronate Copo006Cymer Mixed with Hydroxyapatite for Use in Traumatic Bone Defects" Micromachines 12, no. 7: 822. https://doi.org/10.3390/mi12070822

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