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Article

Raman Mapping of Biological Systems Interacting with a Disordered Nanostructured Surface: A Simple and Powerful Approach to the Label-Free Analysis of Single DNA Bases

1
Institute for Microelectronics and Microsystems, National Research Council, IMM-CNR, 00133 Rome, Italy
2
Institute of Translational Pharmacology, National Research Council, IFT-CNR, 00133 Rome, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to the work.
Academic Editor: Lucia Romano
Micromachines 2021, 12(3), 264; https://doi.org/10.3390/mi12030264
Received: 19 February 2021 / Revised: 27 February 2021 / Accepted: 1 March 2021 / Published: 4 March 2021
(This article belongs to the Special Issue Nanostructured Surfaces and Devices for Biomedical Applications)
This article demonstrates the possibility to use a novel powerful approach based on Raman mapping of analyte solutions drop casted on a disordered array of Ag covered silicon nanowires (Ag/SiNWs), to identify the characteristic spectral signal of the four DNA bases, adenine (A), thymine (T), cytosine (C), and guanine (G), at concentration as low as 10 ng/µL, and to study their specific way of interacting with the nanostructured substrate. The results show a distinctive and amplified interaction of guanine, the base that is most susceptible to oxidation, with the nanostructured surface. Our findings explain the recently revealed diverse behaviour of cancer and normal DNA deposited on the same Ag/SiNWs, which is ascribed to mechanical deformation and base lesions present on the oxidised DNA molecule backbone and causes detectable variation in the Raman signal, usable for diagnostic purposes. The notable bio-analytical capability of the presented platform, and its sensitivity to the molecule mechanical conformation at the single-base level, thus provides a new reliable, rapid, label-free DNA diagnostic methodology alternative to more sophisticated and expensive sequencing ones. View Full-Text
Keywords: single DNA bases; raman mapping; silicon nanowires; principal component analysis single DNA bases; raman mapping; silicon nanowires; principal component analysis
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MDPI and ACS Style

Mussi, V.; Ledda, M.; Convertino, A.; Lisi, A. Raman Mapping of Biological Systems Interacting with a Disordered Nanostructured Surface: A Simple and Powerful Approach to the Label-Free Analysis of Single DNA Bases. Micromachines 2021, 12, 264. https://doi.org/10.3390/mi12030264

AMA Style

Mussi V, Ledda M, Convertino A, Lisi A. Raman Mapping of Biological Systems Interacting with a Disordered Nanostructured Surface: A Simple and Powerful Approach to the Label-Free Analysis of Single DNA Bases. Micromachines. 2021; 12(3):264. https://doi.org/10.3390/mi12030264

Chicago/Turabian Style

Mussi, Valentina, Mario Ledda, Annalisa Convertino, and Antonella Lisi. 2021. "Raman Mapping of Biological Systems Interacting with a Disordered Nanostructured Surface: A Simple and Powerful Approach to the Label-Free Analysis of Single DNA Bases" Micromachines 12, no. 3: 264. https://doi.org/10.3390/mi12030264

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