Next Article in Journal
Experimental Validation of Injection Molding Simulations of 3D Microparts and Microstructured Components Using Virtual Design of Experiments and Multi-Scale Modeling
Previous Article in Journal
Design Applicable 3D Microfluidic Functional Units Using 2D Topology Optimization with Length Scale Constraints
Open AccessArticle

Pushing the Limits of Spatial Assay Resolution for Paper-Based Microfluidics Using Low-Cost and High-Throughput Pen Plotter Approach

1
Department of Mechanical Engineering, University of Connecticut, Storrs, CT 06269, USA
2
UConn School of Engineering Professional Education, University of Connecticut, Storrs, CT 06269, USA
3
Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA
4
Institute of Materials Science (IMS), University of Connecticut, 97 North Eagleville Road, Storrs, CT 06269, USA
5
Department of Mechanical Engineering, Koç University, Sariyer, Istanbul 34450, Turkey
6
Koç University Arçelik Research Center for Creative Industries (KUAR), Koç University, Sariyer, Istanbul 34450, Turkey
7
Boğaziçi Institute of Biomedical Engineering, Boğaziçi University, Çengelköy, Istanbul 34684, Turkey
8
Koç University Research Center for Translational Medicine, Koç University, Sariyer, Istanbul 34450, Turkey
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(6), 611; https://doi.org/10.3390/mi11060611
Received: 18 May 2020 / Revised: 17 June 2020 / Accepted: 23 June 2020 / Published: 24 June 2020
(This article belongs to the Section D:Materials and Processing)
To transform from reactive to proactive healthcare, there is an increasing need for low-cost and portable assays to continuously perform health measurements. The paper-based analytical devices could be a potential fit for this need. To miniaturize the multiplex paper-based microfluidic analytical devices and minimize reagent use, a fabrication method with high resolution along with low fabrication cost should be developed. Here, we present an approach that uses a desktop pen plotter and a high-resolution technical pen for plotting high-resolution patterns to fabricate miniaturized paper-based microfluidic devices with hundreds of detection zones to conduct different assays. In order to create a functional multiplex paper-based analytical device, the hydrophobic solution is patterned on the cellulose paper and the reagents are deposited in the patterned detection zones using the technical pens. We demonstrated the effect of paper substrate thickness on the resolution of patterns by investigating the resolution of patterns on a chromatography paper with altered effective thickness. As the characteristics of the cellulose paper substrate such as thickness, resolution, and homogeneity of pore structure affect the obtained patterning resolution, we used regenerated cellulose paper to fabricate detection zones with a diameter as small as 0.8 mm. Moreover, in order to fabricate a miniaturized multiplex paper-based device, we optimized packing of the detection zones. We also showed the capability of the presented method for fabrication of 3D paper-based microfluidic devices with hundreds of detection zones for conducting colorimetric assays. View Full-Text
Keywords: high-resolution; miniaturized paper-based assay; multiplex assays; medical diagnostics; microfluidics; colorimetric analysis high-resolution; miniaturized paper-based assay; multiplex assays; medical diagnostics; microfluidics; colorimetric analysis
Show Figures

Figure 1

MDPI and ACS Style

Amin, R.; Ghaderinezhad, F.; Bridge, C.; Temirel, M.; Jones, S.; Toloueinia, P.; Tasoglu, S. Pushing the Limits of Spatial Assay Resolution for Paper-Based Microfluidics Using Low-Cost and High-Throughput Pen Plotter Approach. Micromachines 2020, 11, 611.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop