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Article

Fusing Artificial Cell Compartments and Lipid Domains Using Optical Traps: A Tool to Modulate Membrane Composition and Phase Behaviour

1
Department of Chemistry, Imperial College London, London W12 0BZ, UK
2
Department of Chemical Engineering, Loughborough University, Leicestershire LE11 3TU, UK
3
Department of Chemical Engineering, Imperial College London, London SW7 2AZ, UK
4
fabriCELL, Imperial College London, London SW7 2AZ, UK
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(4), 388; https://doi.org/10.3390/mi11040388
Received: 6 January 2020 / Revised: 25 March 2020 / Accepted: 28 March 2020 / Published: 7 April 2020
(This article belongs to the Special Issue Optical Trapping and Manipulation: From Fundamentals to Applications)
New technologies for manipulating biomembranes have vast potential to aid the understanding of biological phenomena, and as tools to sculpt novel artificial cell architectures for synthetic biology. The manipulation and fusion of vesicles using optical traps is amongst the most promising due to the level of spatiotemporal control it affords. Herein, we conduct a suite of feasibility studies to show the potential of optical trapping technologies to (i) modulate the lipid composition of a vesicle by delivering new membrane material through fusion events and (ii) manipulate and controllably fuse coexisting membrane domains for the first time. We also outline some noteworthy morphologies and transitions that the vesicle undergoes during fusion, which gives us insight into the mechanisms at play. These results will guide future exploitation of laser-assisted membrane manipulation methods and feed into a technology roadmap for this emerging technology. View Full-Text
Keywords: optical traps; vesicles; artificial cells; membrane biophysics; phase separation; membranes optical traps; vesicles; artificial cells; membrane biophysics; phase separation; membranes
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MDPI and ACS Style

Vivek, A.; Bolognesi, G.; Elani, Y. Fusing Artificial Cell Compartments and Lipid Domains Using Optical Traps: A Tool to Modulate Membrane Composition and Phase Behaviour. Micromachines 2020, 11, 388. https://doi.org/10.3390/mi11040388

AMA Style

Vivek A, Bolognesi G, Elani Y. Fusing Artificial Cell Compartments and Lipid Domains Using Optical Traps: A Tool to Modulate Membrane Composition and Phase Behaviour. Micromachines. 2020; 11(4):388. https://doi.org/10.3390/mi11040388

Chicago/Turabian Style

Vivek, Adithya, Guido Bolognesi, and Yuval Elani. 2020. "Fusing Artificial Cell Compartments and Lipid Domains Using Optical Traps: A Tool to Modulate Membrane Composition and Phase Behaviour" Micromachines 11, no. 4: 388. https://doi.org/10.3390/mi11040388

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