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Open AccessArticle

Polarizability-Dependent Sorting of Microparticles Using Continuous-Flow Dielectrophoretic Chromatography with a Frequency Modulation Method

1
Chemical Process Engineering, Faculty of Production Engineering, University of Bremen, Leobener Straße 6, 28359 Bremen, Germany
2
MAPEX Center for Materials and Processes, University of Bremen, 28359 Bremen, Germany
3
Department of Engineering, Brandenburg University of Applied Sciences, Magdeburger Straße 50, 14770 Brandenburg an der Havel, Germany
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(1), 38; https://doi.org/10.3390/mi11010038
Received: 7 November 2019 / Revised: 15 December 2019 / Accepted: 23 December 2019 / Published: 28 December 2019
(This article belongs to the Special Issue Micromachines for Dielectrophoresis)
The separation of microparticles with respect to different properties such as size and material is a research field of great interest. Dielectrophoresis, a phenomenon that is capable of addressing multiple particle properties at once, can be used to perform a chromatographic separation. However, the selectivity of current dielectrophoretic particle chromatography (DPC) techniques is limited. Here, we show a new approach for DPC based on differences in the dielectrophoretic mobilities and the crossover frequencies of polystyrene particles. Both differences are addressed by modulating the frequency of the electric field to generate positive and negative dielectrophoretic movement to achieve multiple trap-and-release cycles of the particles. A chromatographic separation of different particle sizes revealed the voltage dependency of this method. Additionally, we showed the frequency bandwidth influence on separation using one example. The DPC method developed was tested with model particles, but offers possibilities to separate a broad range of plastic and metal microparticles or cells and to overcome currently existing limitations in selectivity. View Full-Text
Keywords: dielectrophoresis (DEP); microparticles; polystyrene; chromatography; interdigitated electrodes; microfluidic; separation dielectrophoresis (DEP); microparticles; polystyrene; chromatography; interdigitated electrodes; microfluidic; separation
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MDPI and ACS Style

Giesler, J.; Pesch, G.R.; Weirauch, L.; Schmidt, M.-P.; Thöming, J.; Baune, M. Polarizability-Dependent Sorting of Microparticles Using Continuous-Flow Dielectrophoretic Chromatography with a Frequency Modulation Method. Micromachines 2020, 11, 38.

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