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Open AccessArticle

In Vitro Assessment of Biocontrol Effects on Fusarium Head Blight and Deoxynivalenol (DON) Accumulation by DON-Degrading Bacteria

1
Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan
2
Tropical Biosphere Research Center, University of the Ryukyus, 1 Senbaru, Nishihara-cho, Okinawa 903-0213, Japan
3
Central Region Agricultural Research Center, National Agriculture and Food Research Organization, 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8666, Japan
4
Department of Molecular Biology, Tokyo University of Agriculture, 1-1-1 Sakuraga-oka, Setagaya-ku, Tokyo 156-8502, Japan
*
Author to whom correspondence should be addressed.
Toxins 2020, 12(6), 399; https://doi.org/10.3390/toxins12060399
Received: 7 April 2020 / Revised: 1 June 2020 / Accepted: 12 June 2020 / Published: 16 June 2020
(This article belongs to the Special Issue Mycotoxins and Related Fungi in Crops)
Fusarium head blight (FHB) of cereals is a severe disease caused by the Fusarium graminearum species complex. It leads to the accumulation of the mycotoxin deoxynivalenol (DON) in grains and other plant tissues and causes substantial economic losses throughout the world. DON is one of the most troublesome mycotoxins because it is a virulence factor to host plants, including wheat, and exhibits toxicity to plants and animals. To control both FHB and DON accumulation, a biological control approach using DON-degrading bacteria (DDBs) is promising. Here, we performed a disease control assay using an in vitro petri dish test composed of germinated wheat seeds inoculated with F. graminearum (Fg) and DDBs. Determination of both grown leaf lengths and hyphal lesion lengths as a measure of disease severity showed that the inoculation of seeds with the DDBs Devosia sp. strain NKJ1 and Nocardioides spp. strains SS3 or SS4 were protective against the leaf growth inhibition caused by Fg. Furthermore, it was as effective against DON accumulation. The inoculation with strains SS3 or SS4 also reduced the inhibitory effect on leaves treated with 10 µg mL−1 DON solution (without Fg). These results indicate that the DDBs partially suppress the disease by degrading DON. View Full-Text
Keywords: Fusarium graminearum; trichothecene; deoxynivalenol; mycotoxin degradation; phytotoxin; biocontrol; Nocardioides; Devosia Fusarium graminearum; trichothecene; deoxynivalenol; mycotoxin degradation; phytotoxin; biocontrol; Nocardioides; Devosia
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Morimura, H.; Ito, M.; Yoshida, S.; Koitabashi, M.; Tsushima, S.; Camagna, M.; Chiba, S.; Takemoto, D.; Kawakita, K.; Sato, I. In Vitro Assessment of Biocontrol Effects on Fusarium Head Blight and Deoxynivalenol (DON) Accumulation by DON-Degrading Bacteria. Toxins 2020, 12, 399.

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