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In Vitro Toxicological Screening of Stable and Senescing Cultures of Aphanizomenon, Planktothrix, and Raphidiopsis

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Department of Hydrobiology, Institute of Environmental Biology, Faculty of Biology, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
2
Department of Orthopedagogy and Physical Therapy, Ternopil V. Hnatiuk National Pedagogical University, 46027 Ternopil, Ukraine
3
Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, Tykistökatu 6A, 20520 Turku, Finland
4
United Nations Educational, Scientific and Cultural Organization (UNESCO) Chair on Ecohydrology and Applied Ecology, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland
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Department of Environmental Medicine, Poznan University of Medical Sciences, Rokietnicka 8, 60-806 Poznań, Poland
6
Institute of Oceanography, University of Gdańsk, Al. Piłsudskiego 46, 81-378 Gdynia, Poland
*
Authors to whom correspondence should be addressed.
Toxins 2020, 12(6), 400; https://doi.org/10.3390/toxins12060400
Received: 13 April 2020 / Revised: 12 June 2020 / Accepted: 15 June 2020 / Published: 17 June 2020
Toxicity of cyanobacteria is the subject of ongoing research, and a number of toxic metabolites have been described, their biosynthesis pathways have been elucidated, and the mechanism of their action has been established. However, several knowledge gaps still exist, e.g., some strains produce hitherto unknown toxic compounds, while the exact dynamics of exerted toxicity during cyanobacterial growth still requires further exploration. Therefore, the present study investigated the toxicity of extracts of nine freshwater strains of Aphanizomenon gracile, an Aphanizomenon sp. strain isolated from the Baltic Sea, a freshwater strain of Planktothrix agardhii, and two strains of Raphidiopsis raciborskii obtained from 25- and 70-day-old cultures. An in vitro experimental model based on Cyprinus carpio hepatocytes (oxidative stress markers, DNA fragmentation, and serine/threonine protein activity) and brain homogenate (cholinesterase activity) was employed. The studied extracts demonstrated toxicity to fish cells, and in general, all examined extracts altered at least one or more of considered parameters, indicating that they possess, to some degree, toxic potency. Although the time from which the extracts were obtained had a significant importance for the response of fish cells, we observed strong variability between the different strains and species. In some strains, extracts that originated from 25-day-old cultures triggered more harmful effects on fish cells compared to those obtained from 70-day-old cultures, whereas in other strains, we observed the opposite effect or a lack of a significant change. Our study revealed that there was no clear or common pattern regarding the degree of cyanobacterial bloom toxicity at a given stage of development. This means that young cyanobacterial blooms that are just forming can pose an equally toxic threat to aquatic vertebrates and ecosystem functioning as those that are stable or old with a tendency to collapse. This might be largely due to a high variability of strains in the bloom. View Full-Text
Keywords: Aphanizomenon; Planktothrix; Raphidiopsis; cyanobacteria; cyanotoxins; toxicological screening; in vitro experimental model Aphanizomenon; Planktothrix; Raphidiopsis; cyanobacteria; cyanotoxins; toxicological screening; in vitro experimental model
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Wejnerowski, Ł.; Falfushynska, H.; Horyn, O.; Osypenko, I.; Kokociński, M.; Meriluoto, J.; Jurczak, T.; Poniedziałek, B.; Pniewski, F.; Rzymski, P. In Vitro Toxicological Screening of Stable and Senescing Cultures of Aphanizomenon, Planktothrix, and Raphidiopsis. Toxins 2020, 12, 400.

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