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Article

Effective Biodegradation of Aflatoxin B1 Using the Bacillus licheniformis (BL010) Strain

1
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China
2
Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
*
Authors to whom correspondence should be addressed.
Toxins 2018, 10(12), 497; https://doi.org/10.3390/toxins10120497
Received: 22 September 2018 / Revised: 16 November 2018 / Accepted: 20 November 2018 / Published: 26 November 2018
(This article belongs to the Section Mycotoxins)
Aflatoxin B1 (AFB1), a pollutant of agricultural products, has attracted considerable attention in recent years, due to its potential impact on health. In the present study, Bacillus licheniformis (BL010) was demonstrated to efficiently degrade AFB1, reducing over 89.1% of the toxin content within 120 h. A crude enzyme solution of BL010 exhibited the highest degradation level (97.3%) after three induction periods. However, uninduced BL010 bacteria was not capable of reducing AFB1. Furthermore, high performance liquid chromatography (HPLC) analysis showed that while a cell-free extract caused a significant decrease in AFB1 content (93.6%, p < 0.05), cell culture fluid treatment did not significantly degrade AFB1. The biotransformation products of AFB1 were detected and further identified by quadrupole time-of-flight liquid chromatography–mass spectrometry (LC-Q-TOF/MS); these corresponded to a molecular formula of C12H14O4. A sequence analysis of whole BL010 genes with a bioinformatics approach identified the secondary structures of two degrading enzymes (Chia010 and Lac010), providing an important basis for subsequent homology modeling and functional predictions. View Full-Text
Keywords: aflatoxin B1; biodegradation; induced effect; degradation enzymes; bioinformatic analysis aflatoxin B1; biodegradation; induced effect; degradation enzymes; bioinformatic analysis
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MDPI and ACS Style

Wang, Y.; Zhang, H.; Yan, H.; Yin, C.; Liu, Y.; Xu, Q.; Liu, X.; Zhang, Z. Effective Biodegradation of Aflatoxin B1 Using the Bacillus licheniformis (BL010) Strain. Toxins 2018, 10, 497. https://doi.org/10.3390/toxins10120497

AMA Style

Wang Y, Zhang H, Yan H, Yin C, Liu Y, Xu Q, Liu X, Zhang Z. Effective Biodegradation of Aflatoxin B1 Using the Bacillus licheniformis (BL010) Strain. Toxins. 2018; 10(12):497. https://doi.org/10.3390/toxins10120497

Chicago/Turabian Style

Wang, Ye, Haiyang Zhang, Hai Yan, Chunhua Yin, Yang Liu, Qianqian Xu, Xiaolu Liu, and Zhongbao Zhang. 2018. "Effective Biodegradation of Aflatoxin B1 Using the Bacillus licheniformis (BL010) Strain" Toxins 10, no. 12: 497. https://doi.org/10.3390/toxins10120497

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