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Biscuits with No Added Sugar Containing Stevia, Coffee Fibre and Fructooligosaccharides Modifies α-Glucosidase Activity and the Release of GLP-1 from HuTu-80 Cells and Serotonin from Caco-2 Cells after In Vitro Digestion

1
Food Bioscience Group, Department of Bioactivity and Food Analysis, Institute of Food Science Research (CIAL, UAM-CSIC), C/ Nicolás Cabrera, 9, Campus de Cantoblanco, Universidad Autónoma de Madrid, 28049 Madrid, Spain
2
Department of Nutritional and Physiological Chemistry, Christian Doppler Laboratory for Bioactive Aroma Compounds, Faculty of Chemistry, University of Vienna, 1090 Vienna, Austria
*
Author to whom correspondence should be addressed.
Nutrients 2017, 9(7), 694; https://doi.org/10.3390/nu9070694
Received: 27 April 2017 / Revised: 16 June 2017 / Accepted: 28 June 2017 / Published: 4 July 2017
This study assessed the in vitro effects of the bioaccessible food components released during the simulated human digestion of a coffee fibre-containing biscuit (CFB) on α-glucosidase activity, antioxidant capacity and satiety hormones. Digest of CFB presented a significantly (p < 0.05) lower amount of sugar (68.6 mg/g) and a higher antioxidant capacity (15.1 mg chlorogenic acid eq./g) than that of a sucrose-containing biscuit (SCB). The CFB significantly reduced (p < 0.05) α-glucosidase activity (IC50 = 3.3 mg/mL) compared to the SCB (IC50 = 6.2 mg/mL). Serotonin and glucagon-like peptide-1 (GLP-1) release by differentiated Caco-2 and HuTu-80 cells, respectively, was stimulated by the CFB (355% at a concentration of 0.5 mg/mL and 278% at a concentration of 0.05 mg/mL) to the same order of magnitude as those of the SCB. To summarize, the CFB was demonstrated to reduce monosaccharide bioaccessibility, to inhibit a diabetes-related digestive enzyme, and to improve the release of satiety hormones. View Full-Text
Keywords: α-glucosidase; biscuits; coffee fibre; fructoologosaccharides; GLP-1; serotonin; stevia; non-nutritive sweeteners α-glucosidase; biscuits; coffee fibre; fructoologosaccharides; GLP-1; serotonin; stevia; non-nutritive sweeteners
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Martinez-Saez, N.; Hochkogler, C.M.; Somoza, V.; Del Castillo, M.D. Biscuits with No Added Sugar Containing Stevia, Coffee Fibre and Fructooligosaccharides Modifies α-Glucosidase Activity and the Release of GLP-1 from HuTu-80 Cells and Serotonin from Caco-2 Cells after In Vitro Digestion. Nutrients 2017, 9, 694.

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