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Lactobacillus fermentum Suo Attenuates HCl/Ethanol Induced Gastric Injury in Mice through Its Antioxidant Effects

by 1,2,†, 3,4,5,†, 3,4,5,†, 3,4,5, 3,4,5, 6,* and 1,*
1
Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, China
2
College of Food Science, Southwest University, Chongqing 400715, China
3
Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
4
Chongqing Collaborative Innovation Center of Functional Food, Chongqing University of Education, Chongqing 400067, China
5
Chongqing Engineering Technology Research Center for Functional Food, Chongqing University of Education, Chongqing 400067, China
6
Institute of Qinghai-Tibetan Plateau, Southwest University for Nationalities, Chengdu 610041, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Nutrients 2016, 8(3), 155; https://doi.org/10.3390/nu8030155
Received: 24 December 2015 / Revised: 24 February 2016 / Accepted: 1 March 2016 / Published: 10 March 2016
The purpose of the study was to determine the inhibitory effects of Lactobacillus fermentum Suo (LF-Suo) on HCl/ethanol induced gastric injury in ICR (Institute for Cancer Research) mice and explain the mechanism of these effects through the molecular biology activities of LF-Suo. The studied mice were divided into four groups: healthy, injured, LF-Suo-L and LF-Suo-H group. After the LF-Suo intragastric administration, the gastric injury area was reduced compared to the injured group. The serum MOT (motilin), SP (substance P), ET (endothelin) levels of LF-Suo treated mice were lower, and SS (somatostatin), VIP (vasoactive intestinal peptide) levels were higher than the injured group mice. The cytokine IL-6 (interleukin 6), IL-12 (interleukin 12), TNF-α (tumor necrosis factor-α) and IFN-γ (interferon-γ) serum levels were decreased after the LF-Suo treatment. The gastric tissues SOD (superoxide dismutase), GSH-Px (glutathione peroxidase), NO (nitric oxide) and activities of LF-Suo treated mice were increased and MDA (malondialdehyde) activity was decreased compared to the injured group mice. By the RT-PCR assay, LF-Suo raised the occludin, EGF (epidermal growth factor), EGFR (epidermal growth factor receptor), VEGF (vascular endothelial growth factor), Fit-1 (fms-like tyrosine kinase-1), IκB-α (inhibitor kappaB-α), nNOS (neuronal nitric oxide synthase), eNOS (endothelial nitric oxide synthase), Mn-SOD, Cu/Zn-SOD, CAT (catalase) mRNA or protein expressions and reduced the COX-2, NF-κB (nuclear factor kappaB), and iNOS (inducible nitric oxide synthase) expressions in gastric tissues compared to the gastric injured group mice. A high concentration (1.0 × 109 CFU/kg b.w.) of LF-Suo treatment showed stronger anti-gastric injury effects compared to a low concentration of (0.5 × 109 CFU/kg b.w.) of LF-Suo treatment. LF-Suo also showed strong survival in pH 3.0 man-made gastric juice and hydrophobic properties. These results indicate that LF-Suo has potential use as probiotics for its gastric injury treatment effects. View Full-Text
Keywords: Lactobacillus fermentum Suo; gastric injury; ethanol; antioxidant; mice Lactobacillus fermentum Suo; gastric injury; ethanol; antioxidant; mice
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MDPI and ACS Style

Suo, H.; Zhao, X.; Qian, Y.; Sun, P.; Zhu, K.; Li, J.; Sun, B. Lactobacillus fermentum Suo Attenuates HCl/Ethanol Induced Gastric Injury in Mice through Its Antioxidant Effects. Nutrients 2016, 8, 155. https://doi.org/10.3390/nu8030155

AMA Style

Suo H, Zhao X, Qian Y, Sun P, Zhu K, Li J, Sun B. Lactobacillus fermentum Suo Attenuates HCl/Ethanol Induced Gastric Injury in Mice through Its Antioxidant Effects. Nutrients. 2016; 8(3):155. https://doi.org/10.3390/nu8030155

Chicago/Turabian Style

Suo, Huayi; Zhao, Xin; Qian, Yu; Sun, Peng; Zhu, Kai; Li, Jian; Sun, Baozhong. 2016. "Lactobacillus fermentum Suo Attenuates HCl/Ethanol Induced Gastric Injury in Mice through Its Antioxidant Effects" Nutrients 8, no. 3: 155. https://doi.org/10.3390/nu8030155

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