The Anti-Inflammatory Effect of Spray-Dried Plasma Is Mediated by a Reduction in Mucosal Lymphocyte Activation and Infiltration in a Mouse Model of Intestinal Inflammation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Diets
2.2. Mesenteric Lymph Node Cell Isolation
2.3. Cell Staining
2.4. Western Blot Analysis
2.5. Real-Time PCR Analysis
2.6. Statistical Analysis
3. Results
3.1. Lymphocyte Populations
3.2. Cytokine Expression
3.3. Expression of Adhesion Molecules
3.4. Expression of Transcription Factors
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Ingredients | Control Diet (g/kg) | SDP 1 Diet (g/kg) |
---|---|---|
SDP | - | 80.0 |
Corn starch | 199.3 | 272.5 |
Dried skim milk | 530.7 | 376.4 |
Sugar | 100.0 | 100.0 |
Soybean oil | 70.0 | 70.7 |
Cellulose | 50.0 | 50.0 |
AIN-93 VX 2 | 10.0 | 10.0 |
AIN-93 MX 2 | 35.0 | 35.0 |
dl-Methionine | 2.5 | 2.9 |
Choline bitartrate | 2.5 | 2.5 |
Antigen | Cell Subtype | Clone | Source |
---|---|---|---|
CD45 | present in all leukocytes | 30-F11 | eBioscience |
CD4 | Th cells | RM4-5 | eBioscience |
CD25 | activated T lymphocytes | PC61.5 | eBioscience |
Foxp3 | regulatory T lymphocytes | FJK-16s | eBioscience |
CD68 | monocytes | FA-11 | Bio-Rad |
Ly6G | neutrophils | 1A8 | Becton Dickinson |
CD14 | activated neutrophils/monocytes | Sa2-8 | eBioscience |
IL-4 | Th2 lymphocytes | 11B11 | eBioscience |
IFN-γ | Th1 lymphocytes | XMG1.2 | eBioscience |
IL-17 | Th17 lymphocytes | EbioTC11-18H10.1 | eBioscience |
Primer | Forward (5′–3′) | Reverse (5′–3′) | Fragment Size | Accession Number |
---|---|---|---|---|
Foxp3 | TTCCTTCCCAGAGTTCTTCCAC | ATGGCCCATCGGATAAGGGT | 93 bp | NM_001199347.1 |
HPRT1 | TGGATACAGGCCAGACTTTGTT | CAGATTCAACTTGCGCTCATC | 163 bp | NM_013556.2 |
ICAM-1 | CAGTCGCTGTGCTTTGAGAA | GAGGTCTCAGCTCCACACTCT | 98 bp | NM_010493.2 |
IFN-γ | CCTTCTTCAGCAACAGCAAGGCG | CTTGGCGCTGGACCTGTGGG | 87 bp | NM_008337.3 |
IL-1β | TGTGAAATGCCACCTTTTGA | GGTCAAAGGTTTGGAAGCAG | 94 bp | NM_008361.4 |
IL-10 | GGCGCTGTCATCGATTTCTCCCC | TGGCCTTGTAGACACCTTGGTCTT | 102 bp | NM_010548.2 |
Integrin β7 | GTATCAGGAGCTGAGACAGTTGATT | AGTCACAGTGGATGACAGGC | 118 bp | NM_013566.2 |
MAdCAM-1 | CCCATGGCCACAGCTACCTCA | CCCTGGCCCTAGTACCCTAC | 85 bp | NM_013591.2 |
TGF-β | CAGTGGCTGAACCAAGGAGACGG | CCCCGACGTTTGGGGCTGATC | 119 bp | NM_011577.1 |
TNF-α | CCACCACGCTCTTCTGTCTAC | AGGGTCTGGGCCATAGAACT | 103 bp | NM_013693.2 |
VCAM-1 | CCTCGCTAGGTTACACAGTGG | TGGGGGCAACGTTGACATA | 86 bp | NM_011693.3 |
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Pérez-Bosque, A.; Miró, L.; Amat, C.; Polo, J.; Moretó, M. The Anti-Inflammatory Effect of Spray-Dried Plasma Is Mediated by a Reduction in Mucosal Lymphocyte Activation and Infiltration in a Mouse Model of Intestinal Inflammation. Nutrients 2016, 8, 657. https://doi.org/10.3390/nu8100657
Pérez-Bosque A, Miró L, Amat C, Polo J, Moretó M. The Anti-Inflammatory Effect of Spray-Dried Plasma Is Mediated by a Reduction in Mucosal Lymphocyte Activation and Infiltration in a Mouse Model of Intestinal Inflammation. Nutrients. 2016; 8(10):657. https://doi.org/10.3390/nu8100657
Chicago/Turabian StylePérez-Bosque, Anna, Lluïsa Miró, Concepció Amat, Javier Polo, and Miquel Moretó. 2016. "The Anti-Inflammatory Effect of Spray-Dried Plasma Is Mediated by a Reduction in Mucosal Lymphocyte Activation and Infiltration in a Mouse Model of Intestinal Inflammation" Nutrients 8, no. 10: 657. https://doi.org/10.3390/nu8100657
APA StylePérez-Bosque, A., Miró, L., Amat, C., Polo, J., & Moretó, M. (2016). The Anti-Inflammatory Effect of Spray-Dried Plasma Is Mediated by a Reduction in Mucosal Lymphocyte Activation and Infiltration in a Mouse Model of Intestinal Inflammation. Nutrients, 8(10), 657. https://doi.org/10.3390/nu8100657