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Article

Effects of Exogenous Dietary Advanced Glycation End Products on the Cross-Talk Mechanisms Linking Microbiota to Metabolic Inflammation

1
Department of Clinical and Biological Sciences, University of Turin, 10125 Turin, Italy
2
Department of Drug Science and Technology, University of Turin, 10125 Turin, Italy
3
Fondazione Edmund Mach, 38010 San Michele all’Adige, Italy
4
Biomedical Sciences, National University of Ireland, H91 TK33 Galway, Ireland
5
Department of Neuroscience, University of Turin, 10124 Turin, Italy
6
Chair of Food Chemistry, Technische Universität Dresden, 01062 Dresden, Germany
7
Pharmacology Unit, School of Pharmacy, University of Camerino, 62032 Camerino, Italy
*
Authors to whom correspondence should be addressed.
Both authors contributed equally.
Nutrients 2020, 12(9), 2497; https://doi.org/10.3390/nu12092497
Received: 29 July 2020 / Revised: 15 August 2020 / Accepted: 17 August 2020 / Published: 19 August 2020
Heat-processed diets contain high amounts of advanced glycation end products (AGEs). Here we explore the impact of an AGE-enriched diet on markers of metabolic and inflammatory disorders as well as on gut microbiota composition and plasma proteins glycosylation pattern. C57BL/6 mice were allocated into control diet (CD, n = 15) and AGE-enriched diet (AGE-D, n = 15) for 22 weeks. AGE-D was prepared replacing casein by methylglyoxal hydroimidazolone-modified casein. AGE-D evoked increased insulin and a significant reduction of GIP/GLP-1 incretins and ghrelin plasma levels, altered glucose tolerance, and impaired insulin signaling transduction in the skeletal muscle. Moreover, AGE-D modified the systemic glycosylation profile, as analyzed by lectin microarray, and increased Nε-carboxymethyllysine immunoreactivity and AGEs receptor levels in ileum and submandibular glands. These effects were associated to increased systemic levels of cytokines and impaired gut microbial composition and homeostasis. Significant correlations were recorded between changes in bacterial population and in incretins and inflammatory markers levels. Overall, our data indicates that chronic exposure to dietary AGEs lead to a significant unbalance in incretins axis, markers of metabolic inflammation, and a reshape of both the intestinal microbiota and plasma protein glycosylation profile, suggesting intriguing pathological mechanisms underlying AGEs-induced metabolic derangements. View Full-Text
Keywords: advanced glycation end products; proteins glycosylation; gut microbiota; metabolic inflammation; insulin signal pathway advanced glycation end products; proteins glycosylation; gut microbiota; metabolic inflammation; insulin signal pathway
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MDPI and ACS Style

Mastrocola, R.; Collotta, D.; Gaudioso, G.; Le Berre, M.; Cento, A.S.; Ferreira Alves, G.; Chiazza, F.; Verta, R.; Bertocchi, I.; Manig, F.; Hellwig, M.; Fava, F.; Cifani, C.; Aragno, M.; Henle, T.; Joshi, L.; Tuohy, K.; Collino, M. Effects of Exogenous Dietary Advanced Glycation End Products on the Cross-Talk Mechanisms Linking Microbiota to Metabolic Inflammation. Nutrients 2020, 12, 2497. https://doi.org/10.3390/nu12092497

AMA Style

Mastrocola R, Collotta D, Gaudioso G, Le Berre M, Cento AS, Ferreira Alves G, Chiazza F, Verta R, Bertocchi I, Manig F, Hellwig M, Fava F, Cifani C, Aragno M, Henle T, Joshi L, Tuohy K, Collino M. Effects of Exogenous Dietary Advanced Glycation End Products on the Cross-Talk Mechanisms Linking Microbiota to Metabolic Inflammation. Nutrients. 2020; 12(9):2497. https://doi.org/10.3390/nu12092497

Chicago/Turabian Style

Mastrocola, Raffaella, Debora Collotta, Giulia Gaudioso, Marie Le Berre, Alessia S. Cento, Gustavo Ferreira Alves, Fausto Chiazza, Roberta Verta, Ilaria Bertocchi, Friederike Manig, Michael Hellwig, Francesca Fava, Carlo Cifani, Manuela Aragno, Thomas Henle, Lokesh Joshi, Kieran Tuohy, and Massimo Collino. 2020. "Effects of Exogenous Dietary Advanced Glycation End Products on the Cross-Talk Mechanisms Linking Microbiota to Metabolic Inflammation" Nutrients 12, no. 9: 2497. https://doi.org/10.3390/nu12092497

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