Next Article in Journal
Sodium and Potassium Intake Assessed by Spot and 24-h Urine in the Population-Based Tromsø Study 2015–2016
Previous Article in Journal
Coffee Abundant in Chlorogenic Acids Reduces Abdominal Fat in Overweight Adults: A Randomized, Double-Blind, Controlled Trial
Article

The Role of Mitochondria in Sex-Dependent Differences in Hepatic Steatosis and Oxidative Stress in Response to Cafeteria Diet-Induced Obesity in Mice

1
Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil
2
Department of Morphophysiological Sciences, State University of Maringá, Maringá 87020-900, Paraná, Brazil
*
Author to whom correspondence should be addressed.
J.M.M. and F.R.S.G. contributed equally and should be considered co-first authors.
Nutrients 2019, 11(7), 1618; https://doi.org/10.3390/nu11071618
Received: 10 May 2019 / Revised: 3 June 2019 / Accepted: 11 July 2019 / Published: 16 July 2019
Female mice fed a cafeteria diet (FCaf) develop higher liver steatosis and oxidative stress than males (MCaf) as a consequence of unresolved ER stress. Here, we investigated whether mitochondria play a role in this sex difference. The isolated mitochondria from FCaf showed more signs of oxidative stress than those of MCaf, correlated with a reduced content of GSH, increased amount of reactive oxygen species (ROS), and lower activities of enzymes involved in ROS neutralisation. Mitochondria from FCaf and MCaf livers exhibited lower rates of succinate-driven state III respiration and reduced ATPase activity in intact coupled mitochondria compared to their controls fed a standard diet (FC and MC), with no differences between the sexes. Fatty acid oxidation in mitochondria and peroxisomes was higher in MCaf and FCaf compared to their respective controls. In the intact perfused liver, there was no difference between sex or diet regarding the fatty acid oxidation rate. These results indicated that cafeteria diet did not affect mitochondrial energy metabolism, even in FCaf livers, which have higher steatosis and cellular oxidative stress. Nevertheless, the increase in mitochondrial ROS generation associated with a decrease in the antioxidant defence capacity, probably contributes to inducing or reinforcing the ER stress in FCaf livers. View Full-Text
Keywords: cafeteria diet; oxidative stress; mitochondrial energy metabolism; ER stress cafeteria diet; oxidative stress; mitochondrial energy metabolism; ER stress
Show Figures

Graphical abstract

MDPI and ACS Style

Morais Mewes, J.; Rodrigues Silva Gasparin, F.; Yoshida, T.; Amâncio Daniel da Silva, M.; Raquel Marçal Natali, M.; Francisco Veiga Bizerra, P.; Sayuri Utsunomiya, K.; Hideo Gilglioni, E.; Shigueaki Mito, M.; Cristiane Mantovanelli, G.; Thais Lima de Souza, B.; Makiyama Klosowski, E.; Luiza Ishii-Iwamoto, E.; Constantin, J.; Polimeni Constantin, R. The Role of Mitochondria in Sex-Dependent Differences in Hepatic Steatosis and Oxidative Stress in Response to Cafeteria Diet-Induced Obesity in Mice. Nutrients 2019, 11, 1618. https://doi.org/10.3390/nu11071618

AMA Style

Morais Mewes J, Rodrigues Silva Gasparin F, Yoshida T, Amâncio Daniel da Silva M, Raquel Marçal Natali M, Francisco Veiga Bizerra P, Sayuri Utsunomiya K, Hideo Gilglioni E, Shigueaki Mito M, Cristiane Mantovanelli G, Thais Lima de Souza B, Makiyama Klosowski E, Luiza Ishii-Iwamoto E, Constantin J, Polimeni Constantin R. The Role of Mitochondria in Sex-Dependent Differences in Hepatic Steatosis and Oxidative Stress in Response to Cafeteria Diet-Induced Obesity in Mice. Nutrients. 2019; 11(7):1618. https://doi.org/10.3390/nu11071618

Chicago/Turabian Style

Morais Mewes, Juliana, Fabiana Rodrigues Silva Gasparin, Tiago Yoshida, Mariana Amâncio Daniel da Silva, Maria Raquel Marçal Natali, Paulo Francisco Veiga Bizerra, Karina Sayuri Utsunomiya, Eduardo Hideo Gilglioni, Marcio Shigueaki Mito, Gislaine Cristiane Mantovanelli, Byanca Thais Lima de Souza, Eduardo Makiyama Klosowski, Emy Luiza Ishii-Iwamoto, Jorgete Constantin, and Rodrigo Polimeni Constantin. 2019. "The Role of Mitochondria in Sex-Dependent Differences in Hepatic Steatosis and Oxidative Stress in Response to Cafeteria Diet-Induced Obesity in Mice" Nutrients 11, no. 7: 1618. https://doi.org/10.3390/nu11071618

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop