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SWATH Differential Abundance Proteomics and Cellular Assays Show In Vitro Anticancer Activity of Arachidonic Acid- and Docosahexaenoic Acid-Based Monoacylglycerols in HT-29 Colorectal Cancer Cells

1
Food Technology Division, Agrifood Campus of International Excellence, ceiA3, University of Almería, 40120 Almería, Spain
2
Proteomics Unit, IMIBIC, Reina Sofía University Hospital, University of Córdoba, 14004 Córdoba, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Nutrients 2019, 11(12), 2984; https://doi.org/10.3390/nu11122984
Received: 23 October 2019 / Revised: 2 December 2019 / Accepted: 4 December 2019 / Published: 6 December 2019
Colorectal cancer (CRC) is one of the most common and mortal types of cancer. There is increasing evidence that some polyunsaturated fatty acids (PUFAs) exercise specific inhibitory actions on cancer cells through different mechanisms, as a previous study on CRC cells demonstrated for two very long-chain PUFA. These were docosahexaenoic acid (DHA, 22:6n3) and arachidonic acid (ARA, 20:4n6) in the free fatty acid (FFA) form. In this work, similar design and technology have been used to investigate the actions of both DHA and ARA as monoacylglycerol (MAG) molecules, and results have been compared with those obtained using the corresponding FFA. Cell assays revealed that ARA- and DHA-MAG exercised dose- and time-dependent antiproliferative actions, with DHA-MAG acting on cancer cells more efficiently than ARA-MAG. Sequential window acquisition of all theoretical mass spectra (SWATH)—mass spectrometry massive quantitative proteomics, validated by parallel reaction monitoring and followed by pathway analysis, revealed that DHA-MAG had a massive effect in the proteasome complex, while the ARA-MAG main effect was related to DNA replication. Prostaglandin synthesis also resulted as inhibited by DHA-MAG. Results clearly demonstrated the ability of both ARA- and DHA-MAG to induce cell death in colon cancer cells, which suggests a direct relationship between chemical structure and antitumoral actions. View Full-Text
Keywords: colorectal cancer; proteomics; SWATH; docosahexaenoic acid; arachidonic acid; HT-29 cells; monoacylglycerols colorectal cancer; proteomics; SWATH; docosahexaenoic acid; arachidonic acid; HT-29 cells; monoacylglycerols
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MDPI and ACS Style

González-Fernández, M.J.; Fabrikov, D.; Ramos-Bueno, R.P.; Guil-Guerrero, J.L.; Ortea, I. SWATH Differential Abundance Proteomics and Cellular Assays Show In Vitro Anticancer Activity of Arachidonic Acid- and Docosahexaenoic Acid-Based Monoacylglycerols in HT-29 Colorectal Cancer Cells. Nutrients 2019, 11, 2984. https://doi.org/10.3390/nu11122984

AMA Style

González-Fernández MJ, Fabrikov D, Ramos-Bueno RP, Guil-Guerrero JL, Ortea I. SWATH Differential Abundance Proteomics and Cellular Assays Show In Vitro Anticancer Activity of Arachidonic Acid- and Docosahexaenoic Acid-Based Monoacylglycerols in HT-29 Colorectal Cancer Cells. Nutrients. 2019; 11(12):2984. https://doi.org/10.3390/nu11122984

Chicago/Turabian Style

González-Fernández, María J., Dmitri Fabrikov, Rebeca P. Ramos-Bueno, José L. Guil-Guerrero, and Ignacio Ortea. 2019. "SWATH Differential Abundance Proteomics and Cellular Assays Show In Vitro Anticancer Activity of Arachidonic Acid- and Docosahexaenoic Acid-Based Monoacylglycerols in HT-29 Colorectal Cancer Cells" Nutrients 11, no. 12: 2984. https://doi.org/10.3390/nu11122984

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