Unravelling Bile Viromes of Free-Range Laying Chickens Clinically Diagnosed with Spotty Liver Disease: Emergence of Many Novel Chaphamaparvoviruses into Multiple Lineages
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling and Ethical Approval
2.2. Virus Enrichment and Virus Nucleic Acid Extraction
2.3. Next-Generation Sequencing
2.4. Bioinformatic Analyses
2.5. Comparative Genomics and Phylogenetic Analyses
3. Results
3.1. Genomic Characteristic and Diversity of Sequenced GaChPV
3.2. Comparative Analyses of Coding Genes of GaChPV
3.3. Emergence of GaChPV into Multiple Lineages
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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SL | GaChPV (GenBank Accession No.) | Length (nt) | Best BlastN Match (Organism/Query Coverage (%)/E-Value/GenBank Accession No.) | nt Similarity (%) |
---|---|---|---|---|
1 | GaChPV-4 (OM920501) | 4367 | Galliform chaphamaparvovirus 2/97/0.0/MG846442.1 | 79.90 |
2 | GaChPV-5 (OM920502) | 4311 | Galliform chaphamaparvovirus 3/63/0.0/MW306779.1 | 74.90 |
3 | GaChPV-6 (OM920503) | 4270 | Peafowl parvovirus 2/33/3.00 × 10−44/MK988620.1 | 64.11 |
4 | GaChPV-7 (OM920504) | 4230 | Peafowl parvovirus 1/29/1.00 × 10−125/MK988619.1 | 73.95 |
5 | GaChPV-8 (OM920505) | 4225 | Chestnut teal chaphamaparvovirus 1/87/0/MT247758.1 | 73.73 |
6 | GaChPV-9 (OM920506) | 4212 | Pavo cristatus parvoviridae sp./30/0/MW046349.1 | 76.56 |
7 | GaChPV-10 (OM920507) | 4211 | Peafowl parvovirus 1/36/6.00 × 10−79/MK988619.1 | 71.12 |
8 | GaChPV-11 (OM920508) | 4070 | Cygnus columbianus Chaphamaparvovirus/19/4.00 × 10−30/MW046623.1 | 76.22 |
9 | GaChPV-2 (OM920509) | 4032 | Galliform chaphamaparvovirus 2/97/0.0/MG846443.1 | 87.39 |
10 | GaChPV-12 (OM920510) | 3429 | Peafowl parvovirus 2/67/2.00 × 10−127/MK988620.1 | 71.15 |
11 | GaChPV-13 (OM920511) | 2582 | Peafowl parvovirus 2/30/6.00 × 10−62/MK988620.1 | 73.30 |
12 | GaChPV-14 (OM920512) | 1948 | Parvoviridae sp./19/6.00 × 10−17/MT138323.1 | 67.62 |
13 | GaChPV-15 (OM920513) | 1914 | Ara ararauna Chaphamaparvovirus/52/4.00 × 10−52/MW046364.1 | 71.59 |
14 | GaChPV-16 (OM920514) | 1894 | Pavo cristatus parvoviridae sp./77/0.0/MW046349.1 | 78.66 |
15 | GaChPV-17 (OM920515) | 1622 | Peafowl parvovirus 2/92/0.0/ MK988620.1 | 71.29 |
NS3 | NS2 | NS1 | VP1 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
SL No | Genome (GenBank Accession No) | Gene Coordinate (nt Length) | AA Similarity (%) | Best BLAST Match | Gene Coordinate (nt Length) | AA Similarity (%) | Best BLAST Match | Gene Coordinate (nt Length) | AA Similarity (%) | Best BLAST Match | Gene Coordinate (nt Length) | AA Similarity (%) | Best BLAST Match |
1 | GaChPV-4 (OM920501) | 297–740 (444) | 74.83 | NS3 (GaChV-3, QRK03700.1) | 1917–2525 (609) | 81.14 | GaChV-3, QRK03699.1 | 623–2647 (2025) | 77.63 | GaChV-3, QRK03698.1 | 2644–4317 (1674) | 70.02 | GaChV-3, QRK03701.1 |
2 | GaChPV-5 (OM920502) | 285–728 (444) | 69.39 | NS3 (GaChV-3, QRK03700.1) | 2067–2519 (453) | 72.67 | WDChPV, QMI57952.1 | 611–2629 (2019) | 64.45 | GaChV-3, QRK03698.1 | 2626–4290 (1665) | 59.33 | CTChPV-1, QMI57831.1 |
3 | GaChPV-6 (OM920503) | 343–780 (438) | 44.76 | ORF1 (ChFV, QSH48278.1) | 1906–2580 (675) | 53.63 | PfPV-2, QGJ83205.1 | 684–2684 (2001) | 45.94 | PfPV-2, QGJ83204.1 | 2659–4173 (1515) | 44.04 | PfPV-2, QGJ83206.1 |
4 | GaChPV-7 (OM920504) | 335–778 (444) | 51.35 | ORF1 (ChFV, QSH48278.1) | 1946–2587 (642) | 65.71 | PfPV-1, QGJ83202.1 | 670–2673 (2004) | 58.15 | PfPV-2, QGJ83201.1 | 2666–4216 (1551) | 56.18 | PfPV-1, QGJ83203.1 |
5 | GaChPV-8 (OM920505) | 81–524 (444) | 61.22 | NS3 (GaChV-3, QRK03700.1) | 1695–2312 (618) | 68.91 | CTChPV-1, QMI57830.1 | 407–2434 (2028) | 61.63 | GaChPV-2, AXL64657.1 | 2431–4110 (1680) | 60.34 | DAChPV-1, QRK03681.1 |
6 | GaChPV-9 (OM920506) | 335–778 (444) | 42.86 | HP (PsChPV-1, QZW33714.1) | 1991–2590 (600) | 68.97 | PfPV-1, QGJ83202.1 | 685–2673 (1989) | 59.21 | PfPV-1, QGJ83201.1 | 2666–4135 (1500) | 65.00 | PCPV, QTE03716.1 |
7 | GaChPV-10 (OM920507) | 307–759 (453) | 49.65 | HP (PsChPV-1, QZW33714.1) | 1858–2571 (714) | 64.29 | PfPV-1, QGJ83202.1 | 651–2657 (2007) | 56.29 | PfPV-1, QGJ83201.1 | 2650–4194 (1545) | 53.76 | PfPV-2, QGJ83206.1 |
8 | GaChPV-11 (OM920508) | 145–582 (438) | 45.14 | ORF1 (ChFV, QSH48278.1) | 1708–2382 (675) | 52.75 | AAPV, QTE04008.1 | 474–2486 (2013) | 45.29 | PfPV-2, QGJ83204.1 | 2461–3978 (1518) | 46.18 | PfPV-2, QGJ83206.1 |
9 | GaChPV-2 (OM920509) | 346–789 (444) | 74.15 | NS3 (GaChV-3, QRK03700.1) | 1966–2577 (612) | 78.86 | GaChV-3, QRK03699.1 | 672–2693 (2022) | 88.26 | GaChPV-2, AXL64657.1 | 2690–3997 (1308) | 85.20 | GaChPV-2, AXL64658.1 |
10 | GaChPV-12 (OM920510) | 61–477 (417) | 48.53 | ORF1 (ChFV, QSH48278.1) | 1600–2280 (681) | 67.63 | PfPV-2, QGJ83205.1 | 369–2357 (1989) | 59.16 | PfPV-2, QGJ83204.1 | 2287–3327 (1041) | 61.41 | PfPV-2, QGJ83206.1 |
11 | GaChPV-13 (OM920511) | 8–403 (396) | 54.00 | ORF1 (ChFV, QSH48278.1) | 1583–2209 (627) | 62.43 | PfPV-1, QGJ83202.1 | 292–2292 (2001) | 56.59 | PfPV-1, QGJ83201.1 | 2250–2543 (294) | 63.95 | PCPV, QTE03716.1 |
12 | GaChPV-14 (OM920512) | 2–202 (201) | 36.92 | PfPV-1, QGJ83202.1 | 281–1795 (1515) | 48.04 | RcPV, QKE54986.1 | ||||||
13 | GaChPV-15 (OM920513) | 908–1525 (618) | 63.90 | CTChPV-1, QMI57830.1 | 25–1659 (1635) | 54.73 | DAChPV-2, QRK03694.1 | 1830–1681 (150) | 51.02 | WDChPV, QMI57935.1 | |||
14 | GaChPV-16 (OM920514) | 388–936 (549) | 72.13 | PfPV-1, QGJ83202.1 | 147–1022 (876) | 55.67 | PfPV-1, QGJ83201.1 | 980–1195 (216) | 63.33 | PfPV-2, QGJ83206.1 | |||
1430–1729 (300) | 45.83 | PfPV-1, QGJ83203.1 | |||||||||||
1882–1721 (162) | 56.60 | PfPV-1, QGJ83203.1 | |||||||||||
15 | GaChPV-17 (OM920515) | 498–1181 (684) | 64.16 | PfPV-2, QGJ83205.1 | 74–1258 (1185) | 56.93 | PfPV-2, QGJ83204.1 | 1227–1586 (360) | 71.17 | PfPV-2, QGJ83206.1 |
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Sarker, S.; Talukder, S.; Anwar, A.; Van, T.T.H.; Petrovski, S. Unravelling Bile Viromes of Free-Range Laying Chickens Clinically Diagnosed with Spotty Liver Disease: Emergence of Many Novel Chaphamaparvoviruses into Multiple Lineages. Viruses 2022, 14, 2543. https://doi.org/10.3390/v14112543
Sarker S, Talukder S, Anwar A, Van TTH, Petrovski S. Unravelling Bile Viromes of Free-Range Laying Chickens Clinically Diagnosed with Spotty Liver Disease: Emergence of Many Novel Chaphamaparvoviruses into Multiple Lineages. Viruses. 2022; 14(11):2543. https://doi.org/10.3390/v14112543
Chicago/Turabian StyleSarker, Subir, Saranika Talukder, Arif Anwar, Thi Thu Hao Van, and Steve Petrovski. 2022. "Unravelling Bile Viromes of Free-Range Laying Chickens Clinically Diagnosed with Spotty Liver Disease: Emergence of Many Novel Chaphamaparvoviruses into Multiple Lineages" Viruses 14, no. 11: 2543. https://doi.org/10.3390/v14112543
APA StyleSarker, S., Talukder, S., Anwar, A., Van, T. T. H., & Petrovski, S. (2022). Unravelling Bile Viromes of Free-Range Laying Chickens Clinically Diagnosed with Spotty Liver Disease: Emergence of Many Novel Chaphamaparvoviruses into Multiple Lineages. Viruses, 14(11), 2543. https://doi.org/10.3390/v14112543