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Article

Enzyme-Linked Phage Receptor Binding Protein Assays (ELPRA) Enable Identification of Bacillus anthracis Colonies

Department of Bacteriology and Toxinology, Bundeswehr Institute of Microbiology (IMB), 80937 Munich, Germany
*
Author to whom correspondence should be addressed.
Both authors contributed equally to this work.
Academic Editors: Joana Azeredo and Sílvio Santos
Viruses 2021, 13(8), 1462; https://doi.org/10.3390/v13081462
Received: 30 June 2021 / Revised: 19 July 2021 / Accepted: 24 July 2021 / Published: 27 July 2021
Bacteriophage receptor binding proteins (RBPs) are employed by viruses to recognize specific surface structures on bacterial host cells. Recombinant RBPs have been utilized for detection of several pathogens, typically as fusions with reporter enzymes or fluorescent proteins. Identification of Bacillus anthracis, the etiological agent of anthrax, can be difficult because of the bacterium’s close relationship with other species of the Bacillus cereussensu lato group. Here, we facilitated the identification of B. anthracis using two implementations of enzyme-linked phage receptor binding protein assays (ELPRA). We developed a single-tube centrifugation assay simplifying the rapid analysis of suspect colonies. A second assay enables identification of suspect colonies from mixed overgrown solid (agar) media derived from the complex matrix soil. Thus, these tests identified vegetative cells of B. anthracis with little processing time and may support or confirm pathogen detection by molecular methods such as polymerase chain reaction. View Full-Text
Keywords: bacteriophage; receptor binding protein; reporter fusions; enzyme-linked phage protein assay; ELPRA; anthrax; Bacillus anthracis bacteriophage; receptor binding protein; reporter fusions; enzyme-linked phage protein assay; ELPRA; anthrax; Bacillus anthracis
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MDPI and ACS Style

Braun, P.; Rupprich, N.; Neif, D.; Grass, G. Enzyme-Linked Phage Receptor Binding Protein Assays (ELPRA) Enable Identification of Bacillus anthracis Colonies. Viruses 2021, 13, 1462. https://doi.org/10.3390/v13081462

AMA Style

Braun P, Rupprich N, Neif D, Grass G. Enzyme-Linked Phage Receptor Binding Protein Assays (ELPRA) Enable Identification of Bacillus anthracis Colonies. Viruses. 2021; 13(8):1462. https://doi.org/10.3390/v13081462

Chicago/Turabian Style

Braun, Peter, Nadja Rupprich, Diana Neif, and Gregor Grass. 2021. "Enzyme-Linked Phage Receptor Binding Protein Assays (ELPRA) Enable Identification of Bacillus anthracis Colonies" Viruses 13, no. 8: 1462. https://doi.org/10.3390/v13081462

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