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Goose Nephritic Astrovirus Infection of Goslings Induces Lymphocyte Apoptosis, Reticular Fiber Destruction, and CD8 T-Cell Depletion in Spleen Tissue

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Chao-Nan Lin and Peck Toung Ooi
Viruses 2021, 13(6), 1108; https://doi.org/10.3390/v13061108
Received: 29 April 2021 / Revised: 31 May 2021 / Accepted: 5 June 2021 / Published: 9 June 2021
(This article belongs to the Topic Veterinary Infectious Diseases)
The emergence of a novel goose nephritic astrovirus (GNAstV) has caused economic losses to the Chinese goose industry. High viral load is found in the spleen of goslings infected with GNAstV, but pathological injuries to the spleen due to GNAstV are largely unknown. In this study, 50 two-day-old goslings were infected orally with GNAstV, and 50 goslings were treated with PBS as control. Spleens were collected at different times following infection to assess damage. GNAstV infection caused visceral gout and urate deposition in joints, and resulted in 16% mortality. GNAstV was found in the lymphocytes and macrophages within the spleen. Lymphocyte loss, especially around the white pulp, and destruction and decline in the number of reticular fibers was observed in GNAstV-infected goslings. Moreover, in GNAstV-infected goslings, ultrahistopathological examination found that splenic lymphocytes exhibited condensed chromatin and apoptotic bodies, and reticular cells displayed damage to plasma membrane integrity and swollen mitochondria. Furthermore, TUNEL staining confirmed apoptosis of lymphocytes, and the mRNA levels of Fas and FasL were significantly increased in the GNAstV-infected goslings. In addition, GNAstV infection reduced the number and protein expression of CD8. In conclusion, GNAstV infection causes lymphocyte depletion, reticular cell necrosis, reticular fiber destruction, lymphocyte apoptosis, and reduction in CD8 levels, which contribute to spleen injury. View Full-Text
Keywords: goose nephritic astrovirus; spleen; pathological changes; apoptosis; CD8 goose nephritic astrovirus; spleen; pathological changes; apoptosis; CD8
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MDPI and ACS Style

Ding, R.; Huang, H.; Wang, H.; Yi, Z.; Qiu, S.; Lv, Y.; Bao, E. Goose Nephritic Astrovirus Infection of Goslings Induces Lymphocyte Apoptosis, Reticular Fiber Destruction, and CD8 T-Cell Depletion in Spleen Tissue. Viruses 2021, 13, 1108. https://doi.org/10.3390/v13061108

AMA Style

Ding R, Huang H, Wang H, Yi Z, Qiu S, Lv Y, Bao E. Goose Nephritic Astrovirus Infection of Goslings Induces Lymphocyte Apoptosis, Reticular Fiber Destruction, and CD8 T-Cell Depletion in Spleen Tissue. Viruses. 2021; 13(6):1108. https://doi.org/10.3390/v13061108

Chicago/Turabian Style

Ding, Rui, Han Huang, Hongyu Wang, Zewen Yi, Siyu Qiu, Yingjun Lv, and Endong Bao. 2021. "Goose Nephritic Astrovirus Infection of Goslings Induces Lymphocyte Apoptosis, Reticular Fiber Destruction, and CD8 T-Cell Depletion in Spleen Tissue" Viruses 13, no. 6: 1108. https://doi.org/10.3390/v13061108

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