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Article

Comparison of Throat Washings, Nasopharyngeal Swabs and Oropharyngeal Swabs for Detection of SARS-CoV-2

1
Department of Infection Prevention and Infectious Diseases, University Medical Center Regensburg, 93053 Regensburg, Germany
2
Institute of Medical Microbiology and Hygiene, University of Regensburg, 93053 Regensburg, Germany
3
Institute of Clinical Microbiology and Hygiene, University Medical Center Regensburg, 93053 Regensburg, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Luis Martinez-Sobrido
Viruses 2021, 13(4), 653; https://doi.org/10.3390/v13040653
Received: 29 March 2021 / Revised: 8 April 2021 / Accepted: 8 April 2021 / Published: 10 April 2021
(This article belongs to the Collection SARS-CoV-2 and COVID-19)
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA is detected by reverse-transcription quantitative real-time PCR (RT-qPCR) from respiratory specimens. This study compares throat washings (TW), nasopharyngeal swabs (NS) and oropharyngeal swabs (OS). A total of 102 samples from 34 adult patients with confirmed SARS-CoV-2 infection were analysed by RT-qPCR with absolute quantification. The median concentrations and diagnostic sensitivities were 5.8×104 copies/mL, 85% (NS), 1.4×104, 79% (OS) and 4.3×103, 85% (TW). Concentration differences were significant between NS and TW (P = 0.019). Saliva (SA) was available from 21 patients (median 3.4×103). OS and TW can be considered for SARS-CoV-2 diagnostics, although with slightly lower concentrations. View Full-Text
Keywords: SARS-CoV-2; COVID-19; throat washing; nasopharyngeal swab; oropharyngeal swab; saliva; nucleic acid test; PCR; RT-qPCR; diagnostic sensitivity SARS-CoV-2; COVID-19; throat washing; nasopharyngeal swab; oropharyngeal swab; saliva; nucleic acid test; PCR; RT-qPCR; diagnostic sensitivity
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MDPI and ACS Style

Hitzenbichler, F.; Bauernfeind, S.; Salzberger, B.; Schmidt, B.; Wenzel, J.J. Comparison of Throat Washings, Nasopharyngeal Swabs and Oropharyngeal Swabs for Detection of SARS-CoV-2. Viruses 2021, 13, 653. https://doi.org/10.3390/v13040653

AMA Style

Hitzenbichler F, Bauernfeind S, Salzberger B, Schmidt B, Wenzel JJ. Comparison of Throat Washings, Nasopharyngeal Swabs and Oropharyngeal Swabs for Detection of SARS-CoV-2. Viruses. 2021; 13(4):653. https://doi.org/10.3390/v13040653

Chicago/Turabian Style

Hitzenbichler, Florian, Stilla Bauernfeind, Bernd Salzberger, Barbara Schmidt, and Jürgen J. Wenzel 2021. "Comparison of Throat Washings, Nasopharyngeal Swabs and Oropharyngeal Swabs for Detection of SARS-CoV-2" Viruses 13, no. 4: 653. https://doi.org/10.3390/v13040653

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