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Comparative Susceptibility of Madin–Darby Canine Kidney (MDCK) Derived Cell Lines for Isolation of Swine Origin Influenza A Viruses from Different Clinical Specimens

1
National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3R2, Canada
2
Animal Health Laboratory, University of Guelph, Guelph, ON N1G 2W1, Canada
3
Biovet Inc., Saint-Hyacinthe, QC J2S 8W2, Canada
4
Veterinary Diagnostic Services, Manitoba Agriculture and Resource Development, 545 University Crescent, Winnipeg, MB R3T 5S6, Canada
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Department of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
6
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada
*
Author to whom correspondence should be addressed.
Academic Editors: Chao-Nan Lin and Peck Toung Ooi
Viruses 2021, 13(12), 2346; https://doi.org/10.3390/v13122346
Received: 30 September 2021 / Revised: 15 November 2021 / Accepted: 19 November 2021 / Published: 23 November 2021
(This article belongs to the Topic Veterinary Infectious Diseases)
Madin–Darby canine kidney (MDCK) cells are commonly used for the isolation of mammalian influenza A viruses. The goal of this study was to compare the sensitivity and suitability of the original MDCK cell line in comparison with MDCK-derived cell lines, MDCK.2, MDCK SIAT-1 and MDCK-London for isolation of swine-origin influenza A viruses (IAV-S) from clinical specimens. One-hundred thirty clinical specimens collected from pigs in the form of nasal swabs, lung tissue and oral fluids that were positive by PCR for the presence of IAV-S RNA were inoculated in the cell cultures listed above. MDCK-SIAT1 cells yielded the highest proportion of positive IAV-S isolations from all specimen types. For nasal swabs, 58.62% of the specimens were IAV-S positive in MDCK-SIAT1 cells, followed by MDCK-London (36.21%), and conventional MDCK and MDCK.2 cells (27.5%). For lung specimens, 59.38% were IAV-S positive in MDCK-SIAT1 cells, followed by MDCK-London (40.63%), and conventional MDCK and MDCK.2 cells (18.75–31.25%). Oral fluids yielded the lowest number of positive virus isolation results, but MDCK-SIAT1 cells were still had the highest rate (35%) of IAV-S isolation, whereas the isolation rate in other cells ranged from 5–7.5%. Samples with lower IAV-S PCR cycle threshold (Ct) values were more suitable for culturing and isolation. The isolated IAV-S represented H1N1-β, H1N2-α, H1N1pdm and H3N2 cluster IV and cluster IVB viruses. The result of the current study demonstrated the importance of using the most appropriate MDCK cells when isolating IAV-S from clinical samples. View Full-Text
Keywords: Madin–Darby canine kidney (MDCK) cells; virus isolation; swine influenza; nasal swabs; oral fluids Madin–Darby canine kidney (MDCK) cells; virus isolation; swine influenza; nasal swabs; oral fluids
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MDPI and ACS Style

Suderman, M.; Moniwa, M.; Alkie, T.N.; Ojkic, D.; Broes, A.; Pople, N.; Berhane, Y. Comparative Susceptibility of Madin–Darby Canine Kidney (MDCK) Derived Cell Lines for Isolation of Swine Origin Influenza A Viruses from Different Clinical Specimens. Viruses 2021, 13, 2346. https://doi.org/10.3390/v13122346

AMA Style

Suderman M, Moniwa M, Alkie TN, Ojkic D, Broes A, Pople N, Berhane Y. Comparative Susceptibility of Madin–Darby Canine Kidney (MDCK) Derived Cell Lines for Isolation of Swine Origin Influenza A Viruses from Different Clinical Specimens. Viruses. 2021; 13(12):2346. https://doi.org/10.3390/v13122346

Chicago/Turabian Style

Suderman, Matthew, Mariko Moniwa, Tamiru N. Alkie, Davor Ojkic, Andre Broes, Neil Pople, and Yohannes Berhane. 2021. "Comparative Susceptibility of Madin–Darby Canine Kidney (MDCK) Derived Cell Lines for Isolation of Swine Origin Influenza A Viruses from Different Clinical Specimens" Viruses 13, no. 12: 2346. https://doi.org/10.3390/v13122346

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