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Evaluation of Diagnostic Performance of Three Indirect Enzyme-Linked Immunosorbent Assays for the Detection of IgG Antibodies to Ebola Virus in Human Sera

1
Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases of the National Health Laboratory Service, Sandringham, Johannesburg 2131, South Africa
2
Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2050, South Africa
3
Ministry of Health and Sanitation, Freetown 47235, Sierra Leone
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2019, 11(8), 678; https://doi.org/10.3390/v11080678
Received: 29 May 2019 / Revised: 10 July 2019 / Accepted: 11 July 2019 / Published: 24 July 2019
(This article belongs to the Special Issue Medical Advances in Viral Hemorrhagic Fever Research)
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Abstract

Filovirus serological diagnosis and epidemiological investigations are hampered due to the unavailability of validated immunoassays. Diagnostic performance of three indirect enzyme-linked immunosorbent assays (I-ELISA) was evaluated for the detection of IgG antibody to Ebola virus (EBOV) in human sera. One I-ELISA was based on a whole EBOV antigen (WAg) and two utilized recombinant nucleocapsid (NP) and glycoproteins (GP), respectively. Validation data sets derived from individual sera collected in South Africa (SA), representing an EBOV non-endemic country, and from sera collected during an Ebola disease (EBOD) outbreak in Sierra Leone (SL), were categorized according to the compounded results of the three I-ELISAs and real time reverse-transcription polymerase chain reaction (RT-PCR). At the cut-off values selected at 95% accuracy level by the two-graph receiver operating characteristic analysis, specificity in the SA EBOV negative serum panel (n = 273) ranged from 98.17% (GP ELISA) to 99.27% (WAg ELISA). Diagnostic specificity in the SL EBOV negative panel (n = 676) was 100% by the three ELISAs. The diagnostic sensitivity in 423 RT-PCR confirmed EBOD patients was dependent on the time when the serum was collected after onset of disease. It significantly increased 2 weeks post-onset, reaching 100% sensitivity by WAg and NP and 98.1% by GP I-ELISA. View Full-Text
Keywords: Ebola virus; enzyme-linked immunosorbent assay; whole antigen; nucleocapsid; glycoprotein; IgG antibody; human serum; diagnostic performance Ebola virus; enzyme-linked immunosorbent assay; whole antigen; nucleocapsid; glycoprotein; IgG antibody; human serum; diagnostic performance
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Paweska, J.T.; Moolla, N.; Storm, N.; Msimang, V.; Conteh, O.; Weyer, J.; Vuren, P.J. Evaluation of Diagnostic Performance of Three Indirect Enzyme-Linked Immunosorbent Assays for the Detection of IgG Antibodies to Ebola Virus in Human Sera. Viruses 2019, 11, 678.

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