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Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays

Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus Givat-Ram, Jerusalem 91904, Israel
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2019, 11(7), 632; https://doi.org/10.3390/v11070632
Received: 30 April 2019 / Revised: 23 June 2019 / Accepted: 5 July 2019 / Published: 9 July 2019
(This article belongs to the Section Animal Viruses)
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Abstract

Viroporins are a family of small hydrophobic proteins found in many enveloped viruses that are capable of ion transport. Building upon the ability to inhibit influenza by blocking its archetypical M2 H+ channel, as a family, viroporins may represent a viable target to curb viral infectivity. To this end, using three bacterial assays we analyzed six small hydrophobic proteins from biomedically important viruses as potential viroporin candidates. Our results indicate that Eastern equine encephalitis virus 6k, West Nile virus MgM, Dengue virus 2k, Dengue virus P1, Variola virus gp170, and Variola virus gp151 proteins all exhibit channel activity in the bacterial assays, and as such may be considered viroporin candidates. It is clear that more studies, such as patch clamping, will be needed to characterize the ionic conductivities of these proteins. However, our approach presents a rapid procedure to analyze open reading frames in other viruses, yielding new viroporin candidates for future detailed investigation. Finally, if conductivity is proven vital to their cognate viruses, the bio-assays presented herein afford a simple approach to screen for new channel blockers. View Full-Text
Keywords: viral channels; bacterial assays; channel blockers; anti-viral drugs viral channels; bacterial assays; channel blockers; anti-viral drugs
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Tomar, P.P.S.; Oren, R.; Krugliak, M.; Arkin, I.T. Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays. Viruses 2019, 11, 632.

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