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Viruses 2019, 11(2), 138; https://doi.org/10.3390/v11020138

Subterranean Clover Stunt Virus Revisited: Detection of Two Missing Genome Components

1
Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ), Inhoffenstraße 7B, 38124 Braunschweig, Germany
2
Institute for Integrative Biology of the Cell, UMR9198, CNRS, Université Paris-Sud, CEA, 91198 Gif-sur-Yvette, France
3
Im Spargelfeld 1, 38162 Cremlingen, Germany
4
NSW Department of Primary Industries, Tamworth Agricultural Institute, 4 Marsden Park Road, Calala, NSW 2340, Australia
5
The University of Queensland, QAAFI, Ecosciences Precinct, GPO Box 267, Brisbane, QLD 4001, Australia
6
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
7
22 Megalong Crescent, Harrison, ACT 2914, Australia
*
Author to whom correspondence should be addressed.
Current address: UMR 8030 Genomics Metabolics, Laboratory of Systems and Synthetic Biology iSSB, Univ. Évry, CNRS, CEA, Université Paris-Saclay. Genopole Campus 1, Genavenir 6, 5 rue Henri Desbruères, 91030 Evry CEDEX, France.
Deceased in 2016.
Received: 14 January 2019 / Revised: 28 January 2019 / Accepted: 1 February 2019 / Published: 4 February 2019
(This article belongs to the Special Issue Plant Virus Ecology and Biodiversity)
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Abstract

Subterranean clover stunt virus (SCSV) is a type species of the genus Nanovirus in the family Nanoviridae. It was the first single-stranded DNA plant virus with a multipartite genome, of which genomic DNA sequences had been determined. All nanoviruses have eight genome components except SCSV, for which homologs of two genome components present in all other nanovirus genomes, DNA-U2 and DNA-U4, were lacking. We analysed archived and more recent samples from SCSV-infected legume plants to verify its genome composition and found the missing genome components. These results indicated that SCSV also has eight genome components and is a typical member of the genus Nanovirus. View Full-Text
Keywords: nanovirus; virus evolution; rolling circle replication; high-throughput sequencing nanovirus; virus evolution; rolling circle replication; high-throughput sequencing
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Knierim, D.; Barrière, Q.; Grigoras, I.; Winter, S.; Vetten, H.-J.; Schwinghamer, M.; Thomas, J.; Chu, P.; Gronenborn, B.; Timchenko, T. Subterranean Clover Stunt Virus Revisited: Detection of Two Missing Genome Components. Viruses 2019, 11, 138.

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