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Rapid Detection of Peste des Petits Ruminants Virus (PPRV) Nucleic Acid Using a Novel Low-Cost Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for Future Use in Nascent PPR Eradication Programme
Open AccessArticle

Peste des Petits Ruminants Virus-Like Particles Induce a Potent Humoral and Cellular Immune Response in Goats

1
Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130122, Jilin, China
2
Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg R3E 3R2, Manitoba, Canada
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Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada
4
College of Veterinary Medicine, Jilin Agricultural University, Changchun 130122, Jilin, China
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College of Veterinary Medicine, Huanan Agricultural University, Guangzhou 510642, Guangdong, China
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Xinxiang medical university, Xinxiang 453003, Henan, China
7
College of Veterinary Medicine, Jilin University, Changchun 130122, Jilin, China
*
Authors to whom correspondence should be addressed.
Viruses 2019, 11(10), 918; https://doi.org/10.3390/v11100918
Received: 11 July 2019 / Revised: 30 September 2019 / Accepted: 1 October 2019 / Published: 5 October 2019
Peste des petits ruminants is a highly contagious acute or subacute disease of small ruminants caused by the peste des petits ruminants virus (PPRV), and it is responsible for significant economic losses in animal husbandry. Vaccination represents the most effective means of controlling this disease, with virus-like particle (VLP) vaccines offering promising vaccine candidates. In this study, a PPRV VLP-based vaccine was developed using a baculovirus expression system, allowing for the simultaneous expression of the PPRV matrix (M), hemagglutinin (H), fusion (F) and nucleocapsid (N) proteins in insect cells. Immunization of mice and goats with PPRV VLPs elicited a robust neutralization response and a potent cellular immune response. Mouse studies demonstrated that VLPs induced a more robust IFN-γ response in CD4+ and CD8+ T cells than PPRV Nigeria 75/1 and recruited and/or activated more B cells and dendritic cells in inguinal lymph nodes. In addition, PPRV VLPs induced a strong Th1 class response in mice, as indicated by a high IgG2a to IgG1 ratio. Goat studies demonstrated that PPRV VLPs can induce the production of antibodies specific for F and H proteins and can also stimulate the production of virus neutralizing antibodies to the same magnitude as the PPRV Nigeria 75/1 vaccine. Higher amounts of IFN-γ in VLP-immunized animal serum suggested that VLPs also elicited a cellular immune response in goats. These results demonstrated that VLPs elicit a potent immune response against PPRV infection in small ruminants, making PPRV VLPs a potential candidate for PPRV vaccine development. View Full-Text
Keywords: peste des petits ruminants virus; baculovirus; virus-like particles; humoral response; cellular response peste des petits ruminants virus; baculovirus; virus-like particles; humoral response; cellular response
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Yan, F.; Banadyga, L.; Zhao, Y.; Zhao, Z.; Schiffman, Z.; Huang, P.; Li, E.; Wang, C.; Gao, Y.; Feng, N.; Wang, T.; Wang, H.; Xia, X.; Wang, C.; Yang, S.; Qiu, X. Peste des Petits Ruminants Virus-Like Particles Induce a Potent Humoral and Cellular Immune Response in Goats. Viruses 2019, 11, 918.

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