Interaction of the Mouse Polyomavirus Capsid Proteins with Importins Is Required for Efficient Import of Viral DNA into the Cell Nucleus

Soldatova, Irina; Prilepskaja, Terezie; Abrahamyan, Levon; Forstová, Jitka; Huérfano, Sandra

doi:10.3390/v10040165

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Interaction of the Mouse Polyomavirus Capsid Proteins with Importins Is Required for Efficient Import of Viral DNA into the Cell Nucleus

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Department of Genetics and Microbiology, Faculty of Science, Charles University, Vinicna 5, 12844 Prague 2, Czech Republic

Author to whom correspondence should be addressed.

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Current address: Department of Pathology and Microbiology, Faculty of Veterinary Medicine, University of Montreal, Montreal, J2S 2M2, QС, Canada.

Viruses 2018, 10(4), 165; https://doi.org/10.3390/v10040165

Received: 21 February 2018 / Revised: 22 March 2018 / Accepted: 30 March 2018 / Published: 31 March 2018

(This article belongs to the Section Animal Viruses)

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Abstract

The mechanism used by mouse polyomavirus (MPyV) to overcome the crowded cytosol to reach the nucleus has not been fully elucidated. Here, we investigated the involvement of importin α/β1 mediated transport in the delivery of MPyV genomes into the nucleus. Interactions of the virus with importin β1 were studied by co-immunoprecipitation and proximity ligation assay. For infectivity and nucleus delivery assays, the virus and its capsid proteins mutated in the nuclear localization signals (NLSs) were prepared and produced. We found that at early times post infection, virions bound importin β1 in a time dependent manner with a peak of interactions at 6 h post infection. Mutation analysis revealed that only when the NLSs of both VP1 and VP2/3 were disrupted, virus did not bind efficiently to importin β1 and its infectivity remarkably decreased (by 80%). Nuclear targeting of capsid proteins was improved when VP1 and VP2 were co-expressed. VP1 and VP2 were effectively delivered into the nucleus, even when one of the NLS, either VP1 or VP2, was disrupted. Altogether, our results showed that MPyV virions can use VP1 and/or VP2/VP3 NLSs in concert or individually to bind importins to deliver their genomes into the cell nucleus. View Full-Text

Keywords: mouse polyomavirus; trafficking into the nucleus; importin β1; capsid proteins; nuclear localization signal

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MDPI and ACS Style

Soldatova, I.; Prilepskaja, T.; Abrahamyan, L.; Forstová, J.; Huérfano, S. Interaction of the Mouse Polyomavirus Capsid Proteins with Importins Is Required for Efficient Import of Viral DNA into the Cell Nucleus. Viruses 2018, 10, 165. https://doi.org/10.3390/v10040165

AMA Style

Soldatova I, Prilepskaja T, Abrahamyan L, Forstová J, Huérfano S. Interaction of the Mouse Polyomavirus Capsid Proteins with Importins Is Required for Efficient Import of Viral DNA into the Cell Nucleus. Viruses. 2018; 10(4):165. https://doi.org/10.3390/v10040165

Chicago/Turabian Style

Soldatova, Irina, Terezie Prilepskaja, Levon Abrahamyan, Jitka Forstová, and Sandra Huérfano. 2018. "Interaction of the Mouse Polyomavirus Capsid Proteins with Importins Is Required for Efficient Import of Viral DNA into the Cell Nucleus" Viruses 10, no. 4: 165. https://doi.org/10.3390/v10040165

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Interaction of the Mouse Polyomavirus Capsid Proteins with Importins Is Required for Efficient Import of Viral DNA into the Cell Nucleus

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