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Article

Disruption of Autographa Californica Multiple Nucleopolyhedrovirus ac111 Results in Reduced per os Infectivity in a Host-Dependent Manner

by 1,*, 2, 1 and 1
1
Department of Biology, Zhaoqing University, Zhaoqing 526061, China
2
State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China
*
Author to whom correspondence should be addressed.
Viruses 2018, 10(10), 527; https://doi.org/10.3390/v10100527
Received: 12 August 2018 / Revised: 18 September 2018 / Accepted: 20 September 2018 / Published: 27 September 2018
(This article belongs to the Special Issue Baculovirus Advances and Applications)
The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac111 gene is highly conserved in lepidopteran-specific baculoviruses, and its function in the AcMNPV life cycle is still unknown. To investigate the function of ac111, an ac111-knockout AcMNPV (vAc111KO) was constructed through homologous recombination in Escherichia coli. Viral growth curve analysis and plaque assays showed that the deletion of ac111 had no effect on infectious budded virion production. Quantitative real-time polymerase chain reaction analysis confirmed that viral DNA replication was unaffected in the absence of ac111. Electron microscopy revealed that the ac111 deletion did not affect nucleocapsid assembly, occlusion-derived virion formation, or the embedding of occlusion-derived virions into the occlusion bodies. However, in vivo bioassays showed that although the deletion of ac111 did not affect the per os infectivity of AcMNPV in Spodoptera exigua larvae, it led to an approximately five-fold reduction in infectivity of AcMNPV in Trichoplusia ni larvae, and vAc111KO took approximately 21 h longer to kill Trichoplusia ni larvae than the wild-type viruses. Taken together, our results demonstrated that although ac111 is not essential for virus replication in vitro, it plays an important role in the per os infectivity of AcMNPV in a host-dependent manner. View Full-Text
Keywords: AcMNPV; ac111; BV; ODV; per os infectivity AcMNPV; ac111; BV; ODV; per os infectivity
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MDPI and ACS Style

Li, S.; Li, L.; Zhao, H.; Liu, W. Disruption of Autographa Californica Multiple Nucleopolyhedrovirus ac111 Results in Reduced per os Infectivity in a Host-Dependent Manner. Viruses 2018, 10, 527. https://doi.org/10.3390/v10100527

AMA Style

Li S, Li L, Zhao H, Liu W. Disruption of Autographa Californica Multiple Nucleopolyhedrovirus ac111 Results in Reduced per os Infectivity in a Host-Dependent Manner. Viruses. 2018; 10(10):527. https://doi.org/10.3390/v10100527

Chicago/Turabian Style

Li, Sainan, Lu Li, Haizhou Zhao, and Wenhua Liu. 2018. "Disruption of Autographa Californica Multiple Nucleopolyhedrovirus ac111 Results in Reduced per os Infectivity in a Host-Dependent Manner" Viruses 10, no. 10: 527. https://doi.org/10.3390/v10100527

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