Polyelectrolyte-Functionalized Nanofiber Mats Control the Collection and Inactivation of Escherichia coli
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Chemicals
2.2. Cellulose Nanofiber Mat Fabrication
2.3. Functionalization of Electrospun Cellulose Mats with Polyelectrolytes
2.4. Characterization of Polyelectrolye-Functionalized Electrospun Mats
2.5. Quantification of Bacteria Uptake by Polyelectrolye-Functionalized Electrospun Mats
2.6. Evaluation of Antibacterial Activity of Polyelectrolyte-Functionalized Electrospun Mats
3. Results and Discussion
3.1. Characteristics of Polyelectrolyte-Functionalized Cellulose Nanofiber Mats
3.2. Collection of E. coli K12 by Polyelectrolyte-Functionalized Cellulose Nanofiber Mats
3.3. Inactivation of E. coli K12 by Polyelectrolyte-Functionalized Cellulose Nanofiber Mats
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
BET | Brunauer-Emmett-Teller |
CS | Chitosan |
E. coli K12 | Escherichia coli K12 |
FTIR | Fourier transform infrared spectroscopy |
PAA | Poly(acrylic acid) |
pDADMAC | Polydiallyldimethylammonium chloride |
SEM | Scanning electron microscopy |
XPS | X-ray photoelectron spectroscopy |
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Polyelectrolyte Coating | Average Fiber Diameter (μm) | Contact Angle (°) 1 | XPS 2 (Atomic %) | ||
---|---|---|---|---|---|
C | N | O | |||
N/A | 0.85 ± 0.22 | 35.9 ± 4.8 | 56.2 ± 4.3 | - | 41.6 ± 1.8 |
PAA | 0.75 ± 0.20 | 54.5 ± 9.7 | 56.3 ± 5.6 | - | 42.5 ± 4.6 |
CS | 0.84 ± 0.21 | 69.2 ± 7.4 | 62.5 ± 3.3 | 2.3 ± 1.4 | 35.2 ± 4.0 |
pDADMAC | 0.89 ± 0.32 | 42.2 ± 2.8 | 57.8 ± 1.6 | 1.3 ± 0.9 | 40.9 ± 1.8 |
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Rieger, K.A.; Porter, M.; Schiffman, J.D. Polyelectrolyte-Functionalized Nanofiber Mats Control the Collection and Inactivation of Escherichia coli. Materials 2016, 9, 297. https://doi.org/10.3390/ma9040297
Rieger KA, Porter M, Schiffman JD. Polyelectrolyte-Functionalized Nanofiber Mats Control the Collection and Inactivation of Escherichia coli. Materials. 2016; 9(4):297. https://doi.org/10.3390/ma9040297
Chicago/Turabian StyleRieger, Katrina A., Michael Porter, and Jessica D. Schiffman. 2016. "Polyelectrolyte-Functionalized Nanofiber Mats Control the Collection and Inactivation of Escherichia coli" Materials 9, no. 4: 297. https://doi.org/10.3390/ma9040297