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Open AccessArticle

Bioactive Properties of Nanofibres Based on Concentrated Collagen Hydrolysate Loaded with Thyme and Oregano Essential Oils

1
Division Leather and Footwear Research Institute, National Research and Development Institute for Textiles and Leather, 031215 Bucharest, Romania
2
Faculty of Technology, University of Novi Sad, 21102 Novi Sad, Serbia
3
Center for Research and Eco-Metallurgical Expertise (ECOMET UPB), Politehnica University of Bucharest, 313 Spl. Independentei, 060042 Bucharest, Romania
4
Faculty of Biology, University of Bucharest, 60101 Bucharest, Romania
5
National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Romania
*
Authors to whom correspondence should be addressed.
Materials 2020, 13(7), 1618; https://doi.org/10.3390/ma13071618
Received: 24 February 2020 / Revised: 20 March 2020 / Accepted: 30 March 2020 / Published: 1 April 2020
(This article belongs to the Special Issue Novel Materials for Antimicrobial Application)
This research aimed to obtain biocompatible and antimicrobial nanofibres based on concentrated collagen hydrolysate loaded with thyme or oregano essential oils as a natural alternative to synthesis products. The essential oils were successfully incorporated using electrospinning process into collagen resulting nanofibres with diameter from 471 nm to 580 nm and porous structure. The presence of essential oils in collagen nanofibre mats was confirmed by Attenuated Total Reflectance -Fourier Transform Infrared Spectroscopy (ATR-FTIR), Ultraviolet–visible spectroscopy (UV–VIS) and antimicrobial activity. Scanning Electron Microscopy with Energy Dispersive Spectroscopy analyses allowed evaluating the morphology and constituent elements of the nanofibre networks. Microbiological tests performed against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans showed that the presence of essential oils supplemented the new collagen nanofibres with antimicrobial properties. The biocompatibility of collagen and collagen with essential oils was assessed by in vitro cultivation with NCTC clone 929 of fibroblastic cells and cell viability measurement. The results showed that the collagen and thyme or oregano oil composites have no cytotoxicity up to concentrations of 1000 μg·mL−1 and 500 μg mL−1, respectively. Optimization of electrospinning parameters has led to the obtaining of new collagen electrospun nanofibre mats loaded with essential oils with potential use for wound dressings, tissue engineering or protective clothing. View Full-Text
Keywords: antimicrobial nanofibres; collagen hydrolysate; essential oils; bioactive nanofibres; biofilm eradication; alternative antimicrobials; electrospinning technique antimicrobial nanofibres; collagen hydrolysate; essential oils; bioactive nanofibres; biofilm eradication; alternative antimicrobials; electrospinning technique
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MDPI and ACS Style

Berechet, M.D.; Gaidau, C.; Miletic, A.; Pilic, B.; Râpă, M.; Stanca, M.; Ditu, L.-M.; Constantinescu, R.; Lazea-Stoyanova, A. Bioactive Properties of Nanofibres Based on Concentrated Collagen Hydrolysate Loaded with Thyme and Oregano Essential Oils. Materials 2020, 13, 1618.

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