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Mar. Drugs 2018, 16(10), 391; https://doi.org/10.3390/md16100391

Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements

1
Natural Product Laboratory, Department of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China
2
Agricultural Bioinformatics Key Laboratory of Hubei Province, College of Informatics, Huazhong Agricultural University, Wuhan 430070, Hubei, China
3
College of Biological and Environmental Science, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China
*
Author to whom correspondence should be addressed.
Received: 4 September 2018 / Revised: 23 September 2018 / Accepted: 11 October 2018 / Published: 18 October 2018
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Abstract

Menaquinone (MK) has an important role in human metabolism as an essential vitamin (VK2), which is mainly produced through the fermentation of microorganisms. MK8(H2) was identified to be the main menaquinone from Rhodococcus sp. B7740, a bacterium isolated from the arctic ocean. In this work, MK8(H2) (purity: 99.75%) was collected through a convenient and economic extraction process followed by high-speed countercurrent chromatography (HSCCC) purification. Additionally, high-resolution mass spectrometry (HRMS) was performed for further identification and the hydrogenation position of MK8(H2) (terminal unit) was determined using nuclear magnetic resonance (NMR) for the first time. MK8(H2) showed a superior antioxidant effect and antiglycation capacity compared with ubiquinone Q10 and MK4. High-performance liquid chromatography–mass spectrometer (HPLC-MS/MS) and molecular docking showed the fine interaction between MK8(H2) with methylglyoxal (MGO) and bull serum albumin (BSA), respectively. These properties make MK8(H2) a promising natural active ingredient with future food and medicine applications. View Full-Text
Keywords: isoprenoid quinone; MK8(H2); Rhodococcus sp. B7740 from Arctic ocean; UPLC-HRMS; NMR; antioxidant and antiglycation isoprenoid quinone; MK8(H2); Rhodococcus sp. B7740 from Arctic ocean; UPLC-HRMS; NMR; antioxidant and antiglycation
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Chen, Y.; Mu, Q.; Hu, K.; Chen, M.; Yang, J.; Chen, J.; Xie, B.; Sun, Z. Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements. Mar. Drugs 2018, 16, 391.

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