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Mar. Drugs 2018, 16(10), 350; https://doi.org/10.3390/md16100350

Evaluation of Differentiated Bone Cells Proliferation by Blue Shark Skin Collagen via Biochemical for Bone Tissue Engineering

1
Department of Marine Bio-Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
2
Division of Marine Bioscience, Korea Maritime and Ocean University, Busan 606791, Korea
3
Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang 222005, China
4
Bone and Cartilage Research Unit, Arthropôle Liège, University of Liège, CHU Sart-Tilman, 4000 Liège, Belgium
5
Department of Biomaterials Engineering, Universidad Católica San Antonio de Murcia, 30107 Murcia, Spain
6
Department of Food Science, Cornell University, Ithaca, NY 14853-7201, USA
7
Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation, Ministry of Agriculture, Shanghai 201306, China
These authors equally contributed to this work.
*
Authors to whom correspondence should be addressed.
Received: 22 August 2018 / Revised: 7 September 2018 / Accepted: 17 September 2018 / Published: 25 September 2018
(This article belongs to the Special Issue Collagen from Marine Biological Source and Medical Applications)
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Abstract

Collagen from a marine resource is believed to have more potential activity in bone tissue engineering and their bioactivity depends on biochemical and structural properties. Considering the above concept, pepsin soluble collagen (PSC) and acid soluble collagen (ASC) from blue shark (Prionace glauca) skin were extracted and its biochemical and osteogenic properties were investigated. The hydroxyproline content was higher in PSC than ASC and the purified collagens contained three distinct bands α1, α2, and β dimer. The purity of collagen was confirmed by the RP-HPLC profile and the thermogravimetric data showed a two-step thermal degradation pattern. ASC had a sharp decline in viscosity at 20–30 °C. Scanning electron microscope (SEM) images revealed the fibrillar network structure of collagens. Proliferation rates of the differentiated mouse bone marrow-mesenchymal stem (dMBMS) and differentiated osteoblastic (dMC3T3E1) cells were increased in collagen treated groups rather than the controls and the effect was dose-dependent, which was further supported by higher osteogenic protein and mRNA expression in collagen treated bone cells. Among two collagens, PSC had significantly increased dMBMS cell proliferation and this was materialized through increasing RUNX2 and collagen-I expression in bone cells. Accordingly, the collagens from blue shark skin with excellent biochemical and osteogenic properties could be a suitable biomaterial for therapeutic application. View Full-Text
Keywords: blue shark collagen; osteogenic activity; Runx2; differentiated mesenchymal stem cell; osteoblast; proliferation blue shark collagen; osteogenic activity; Runx2; differentiated mesenchymal stem cell; osteoblast; proliferation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Elango, J.; Lee, J.W.; Wang, S.; Henrotin, Y.; de Val, J.E.M.S.; M. Regenstein, J.; Lim, S.Y.; Bao, B.; Wu, W. Evaluation of Differentiated Bone Cells Proliferation by Blue Shark Skin Collagen via Biochemical for Bone Tissue Engineering. Mar. Drugs 2018, 16, 350.

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