Current Issues in Molecular Biology, Volume 27, Issue 1

The history of DNA sequencing dates back to 1970s. During this period the two first generation nucleotide sequencing techniques were developed. Subsequently, Sanger's dideoxy method of sequencing gained popularity over Maxam and Gilbert's chemical me...

In recent decades, next-generation sequencing (NGS) was developed and brought biology into a new era. Rice, maize, wheat, sorghum and barley are the most important cereal crops and feed most of the world's population. Great progress in the study of c...

Next-generation sequencing (NGS) technologies are revolutionizing biology by permitting transcriptome sequencing, whole-genome sequencing and resequencing, and genome-wide single nucleotide polymorphism profiling. Orchid research has benefited from t...

The sudden exponential increase of biological data concerning genome structure and functionalities, also fostered by the advent of next-generation sequencing technologies, while expanding the opportunity to highlight still uncovered molecular aspects...

Bioinformatics encompasses many tools and techniques that today are essential for all areas of research in the biological sciences. New databases with a wealth of information about genomes, proteins, metabolites, and metabolic pathways appear almost...

Wheat (Triticum aestivum L.) is one of the top three global food security crops. Fusarium head blight is one of the major constraints in sustainable wheat production and resistance to the disease is polygenic. This review provides an overview of rece...

Rice (Oryza sativa L.) is a staple food crop for people worldwide, and a key goal has been to increase its grain yield. An increasing population that relies on a decreasing level of farmland has rendered the traditional method for the isolation and u...

RNA-sequencing technology has been widely adopted to investigate host responses during infection with pathogens. Dual RNA-sequencing (RNA-seq) allows the simultaneous capture of pathogen-specific transcripts during infection, providing a more complet...

Reproductive development is a key step of the plant life cycles and indicates the start of a new life cycle. The reproductive organs including flower, fruit and seed, have diverse and complex structures, which is a syndrome in the evolution of angios...

DNA-binding proteins, including transcription factors, epigenetic and chromatin modifiers, control gene expression in plants. To map the binding sites of DNA-binding proteins in the genome is crucial for decoding gene regulatory networks. Chromatin i...