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Volume 27
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10.21775/cimb.027.051
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Current Issues in Molecular Biology is published by MDPI from Volume 43 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Caister Press.
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Review

Advanced Applications of Next-Generation Sequencing Technologies to Orchid Biology

by
Chuan-Ming Yeh
1,†,
Zhong-Jian Liu
2,3,4,† and
Wen-Chieh Tsai
5,6,7,*
1
Division of Strategic Research and Development, Graduate School of Science and Engineering, Satitama University, Saitama, Japan
2
Shenzhen Key Laboratory for Orchid Conservation and Utilization, The National Orchid Conservation Center of China and The Orchid Conservation and Research Center of Shenzhen, Shenzhen, China
3
The Center for Biotechnology and BioMedicine, Graduate School at Shenzhen, Tsinghua University, Shenzhen, China
4
College of Arts, College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, China
5
Institute of Tropical Plant Sciences, National Cheng Kung University, Tainan, Taiwan
6
Department of Life Sciences, National Cheng Kung University, Tainan, Taiwan
7
Orchid Research and Development Center, National Cheng Kung University, Tainan, Taiwan
*
Author to whom correspondence should be addressed.
†
These authors contributed equally.
Curr. Issues Mol. Biol. 2018, 27(1), 51-70; https://doi.org/10.21775/cimb.027.051
Submission received: 5 June 2017 / Revised: 3 July 2017 / Accepted: 4 August 2017 / Published: 8 September 2017

Abstract

Next-generation sequencing (NGS) technologies are revolutionizing biology by permitting transcriptome sequencing, whole-genome sequencing and resequencing, and genome-wide single nucleotide polymorphism profiling. Orchid research has benefited from this breakthrough, and a few orchid genomes are now available; new biological questions can be approached and new breeding strategies can be designed. The first part of this review describes the unique features of orchid biology. The second part provides an overview of the current NGS platforms, many of which are already used in plant laboratories. The third part summarizes the state of orchid transcriptome and genome sequencing and illustrates current achievements. The genetic sequences currently obtained will not only provide a broad scope for the study of orchid biology, but also serves as a starting point for uncovering the mystery of orchid evolution.

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MDPI and ACS Style

Yeh, C.-M.; Liu, Z.-J.; Tsai, W.-C. Advanced Applications of Next-Generation Sequencing Technologies to Orchid Biology. Curr. Issues Mol. Biol. 2018, 27, 51-70. https://doi.org/10.21775/cimb.027.051

AMA Style

Yeh C-M, Liu Z-J, Tsai W-C. Advanced Applications of Next-Generation Sequencing Technologies to Orchid Biology. Current Issues in Molecular Biology. 2018; 27(1):51-70. https://doi.org/10.21775/cimb.027.051

Chicago/Turabian Style

Yeh, Chuan-Ming, Zhong-Jian Liu, and Wen-Chieh Tsai. 2018. "Advanced Applications of Next-Generation Sequencing Technologies to Orchid Biology" Current Issues in Molecular Biology 27, no. 1: 51-70. https://doi.org/10.21775/cimb.027.051

APA Style

Yeh, C.-M., Liu, Z.-J., & Tsai, W.-C. (2018). Advanced Applications of Next-Generation Sequencing Technologies to Orchid Biology. Current Issues in Molecular Biology, 27(1), 51-70. https://doi.org/10.21775/cimb.027.051

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