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Review

Real-Time PCR in Food Science: PCR Diagnostics

by
David Rodríguez-Lázaro
1,*,
Nigel Cook
2 and
Marta Hernández
3
1
Microbiology Section, University of Burgos, Burgos, Spain
2
Food and Environment Research Agency (FERA), York, UK
3
Instituto Tecnológico Agrario de Castilla y León (ITACyL), Valladolid, Spain
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2013, 15(2), 39-44; https://doi.org/10.21775/cimb.015.039
Submission received: 11 September 2012 / Revised: 5 November 2012 / Accepted: 8 January 2013 / Published: 19 March 2013

Abstract

A principal consumer demand is a guarantee of the safety and quality of food. The presence of foodborne pathogens and their potential hazard, the use of genetically modified organisms (GMOs) in food production, and the correct labelling in foods suitable for vegetarians are among the subjects where society demands total transparency. The application of controls within the quality assessment programmes of the food industry is a way to satisfy these demands, and is necessary to ensure efficient analytical methodologies are possessed and correctly applied by the Food Sector. The use of real-time PCR has become a promising alternative approach in food diagnostics. It possesses a number of advantages over conventional culturing approaches, including rapidity, excellent analytical sensitivity and selectivity, and potential for quantification. However, the use of expensive equipment and reagents, the need for qualified personnel, and the lack of standardized protocols are impairing its practical implementation for food monitoring and control.
Keywords: food industry; foodborne pathogens; genetically modified organisms; GMO; labelling of food; real-time PCR; food diagnostics; food monitoring food industry; foodborne pathogens; genetically modified organisms; GMO; labelling of food; real-time PCR; food diagnostics; food monitoring

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MDPI and ACS Style

Rodríguez-Lázaro, D.; Cook, N.; Hernández, M. Real-Time PCR in Food Science: PCR Diagnostics. Curr. Issues Mol. Biol. 2013, 15, 39-44. https://doi.org/10.21775/cimb.015.039

AMA Style

Rodríguez-Lázaro D, Cook N, Hernández M. Real-Time PCR in Food Science: PCR Diagnostics. Current Issues in Molecular Biology. 2013; 15(2):39-44. https://doi.org/10.21775/cimb.015.039

Chicago/Turabian Style

Rodríguez-Lázaro, David, Nigel Cook, and Marta Hernández. 2013. "Real-Time PCR in Food Science: PCR Diagnostics" Current Issues in Molecular Biology 15, no. 2: 39-44. https://doi.org/10.21775/cimb.015.039

APA Style

Rodríguez-Lázaro, D., Cook, N., & Hernández, M. (2013). Real-Time PCR in Food Science: PCR Diagnostics. Current Issues in Molecular Biology, 15(2), 39-44. https://doi.org/10.21775/cimb.015.039

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