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Article

Polyoxazolines with a Vicinally Double-Bioactivated Terminus for Biomacromolecular Affinity Assessment

1
Department of Functional Materials in Medicine and Dentistry, Institute of Functional Materials and Biofabrication and Bavarian Polymer Institute, University of Würzburg, Pleicherwall 2, 97070 Würzburg, Germany
2
Institute of Organic Chemistry, University of Würzburg, Am Hubland, 97070 Würzburg, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Paolo Facci
Sensors 2021, 21(9), 3153; https://doi.org/10.3390/s21093153
Received: 11 February 2021 / Revised: 24 March 2021 / Accepted: 25 March 2021 / Published: 1 May 2021
(This article belongs to the Special Issue Enabling Technologies for Biosensors)
Interactions between proteins and carbohydrates with larger biomacromolecules, e.g., lectins, are usually examined using self-assembled monolayers on target gold surfaces as a simplified model measuring setup. However, most of those measuring setups are either limited to a single substrate or do not allow for control over ligand distance and spacing. Here, we develop a synthetic strategy, consisting of a cascade of a thioesterification, native chemical ligation (NCL) and thiol-ene reaction, in order to create three-component polymer conjugates with a defined double bioactivation at the chain end. The target architecture is the vicinal attachment of two biomolecule residues to the α telechelic end point of a polymer and a thioether group at the ω chain end for fixating the conjugate to a gold sensor chip surface. As proof-of-principle studies for affinity measurements, we demonstrate the interaction between covalently bound mannose and ConA in surface acoustic wave (SAW) and surface plasmon resonance (SPR) experiments. View Full-Text
Keywords: polyoxazolines; functionalization; lectin polyoxazolines; functionalization; lectin
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MDPI and ACS Style

Pinzner, F.; Keller, T.; Mut, J.; Bechold, J.; Seibel, J.; Groll, J. Polyoxazolines with a Vicinally Double-Bioactivated Terminus for Biomacromolecular Affinity Assessment. Sensors 2021, 21, 3153. https://doi.org/10.3390/s21093153

AMA Style

Pinzner F, Keller T, Mut J, Bechold J, Seibel J, Groll J. Polyoxazolines with a Vicinally Double-Bioactivated Terminus for Biomacromolecular Affinity Assessment. Sensors. 2021; 21(9):3153. https://doi.org/10.3390/s21093153

Chicago/Turabian Style

Pinzner, Florian, Thorsten Keller, Jürgen Mut, Julian Bechold, Jürgen Seibel, and Jürgen Groll. 2021. "Polyoxazolines with a Vicinally Double-Bioactivated Terminus for Biomacromolecular Affinity Assessment" Sensors 21, no. 9: 3153. https://doi.org/10.3390/s21093153

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