Next Article in Journal
Machine Learning-Inspired Hybrid Precoding for mmWave MU-MIMO Systems with Domestic Switch Network
Previous Article in Journal
Activity Detection from Electricity Consumption and Communication Usage Data for Monitoring Lonely Deaths
Article

Detecting Effects of Low Levels of FCCP on Stem Cell Micromotion and Wound-Healing Migration by Time-Series Capacitance Measurement

1
Department of Biomedical Engineering, National Yang-Ming University, Taipei 11221, Taiwan
2
Division of Endocrinology and Metabolism, Department of Internal Medicine, Tri-Service General Hospital Songshan Branch, National Defense Medical Center, Taipei 11490, Taiwan
3
Division of Oral and Maxillofacial Surgery, Department of Dentistry, Tri-Service General Hospital, Taipei 11490, Taiwan
4
School of Dentistry, National Defense Medical Center, Taipei 11490, Taiwan
5
Department of Biomedical Engineering, National Defense Medical Center, Taipei 11490, Taiwan
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Spyridon Kintzios
Sensors 2021, 21(9), 3017; https://doi.org/10.3390/s21093017
Received: 6 April 2021 / Revised: 23 April 2021 / Accepted: 23 April 2021 / Published: 25 April 2021
(This article belongs to the Section Biosensors)
Electric cell–substrate impedance sensing (ECIS) has been used as a real-time impedance-based method to quantify cell behavior in tissue culture. The method is capable of measuring both the resistance and capacitance of a cell-covered microelectrode at various AC frequencies. In this study, we demonstrate the application of high-frequency capacitance measurement (f = 40 or 64 kHz) for the sensitive detection of both the micromotion and wound-healing migration of human mesenchymal stem cells (hMSCs). Impedance measurements of cell-covered electrodes upon the challenge of various concentrations of carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), from 0.1 to 30 μM, were conducted using ECIS. FCCP is an uncoupler of mitochondrial oxidative phosphorylation (OXPHOS), thereby reducing mitochondrial ATP production. By numerically analyzing the time-series capacitance data, a dose-dependent decrease in hMSC micromotion and wound-healing migration was observed, and the effect was significantly detected at levels as low as 0.1 μM. While most reported works with ECIS use the resistance/impedance time series, our results suggest the potential use of high-frequency capacitance time series for assessing migratory cell behavior such as micromotion and wound-healing migration. View Full-Text
Keywords: ECIS; high frequency; capacitance; mitochondria; micromotion; wound-healing migration ECIS; high frequency; capacitance; mitochondria; micromotion; wound-healing migration
Show Figures

Figure 1

MDPI and ACS Style

Wang, S.-H.; Tung, T.-H.; Chiu, S.-P.; Chou, H.-Y.; Hung, Y.-H.; Lai, Y.-T.; Lee, Y.-W.; Lee, S.-P.; Lo, C.-M. Detecting Effects of Low Levels of FCCP on Stem Cell Micromotion and Wound-Healing Migration by Time-Series Capacitance Measurement. Sensors 2021, 21, 3017. https://doi.org/10.3390/s21093017

AMA Style

Wang S-H, Tung T-H, Chiu S-P, Chou H-Y, Hung Y-H, Lai Y-T, Lee Y-W, Lee S-P, Lo C-M. Detecting Effects of Low Levels of FCCP on Stem Cell Micromotion and Wound-Healing Migration by Time-Series Capacitance Measurement. Sensors. 2021; 21(9):3017. https://doi.org/10.3390/s21093017

Chicago/Turabian Style

Wang, Si-Han, Tse-Hua Tung, Sheng-Po Chiu, Hsin-Yi Chou, Yu-Han Hung, Yi-Ting Lai, Yu-Wei Lee, Shiao-Pieng Lee, and Chun-Min Lo. 2021. "Detecting Effects of Low Levels of FCCP on Stem Cell Micromotion and Wound-Healing Migration by Time-Series Capacitance Measurement" Sensors 21, no. 9: 3017. https://doi.org/10.3390/s21093017

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop