Next Article in Journal
Data Collection and Analysis Using Wearable Sensors for Monitoring Knee Range of Motion after Total Knee Arthroplasty
Previous Article in Journal
Efficient Data Collection in Widely Distributed Wireless Sensor Networks with Time Window and Precedence Constraints
Open AccessArticle

Long Term Amperometric Recordings in the Brain Extracellular Fluid of Freely Moving Immunocompromised NOD SCID Mice

Chemistry Department, Maynooth University, Maynooth, County Kildare W23 F2H6, Ireland
*
Author to whom correspondence should be addressed.
Academic Editor: Roberto Pilloton
Sensors 2017, 17(2), 419; https://doi.org/10.3390/s17020419
Received: 3 January 2017 / Revised: 10 February 2017 / Accepted: 18 February 2017 / Published: 22 February 2017
(This article belongs to the Section Biosensors)
We describe the in vivo characterization of microamperometric sensors for the real-time monitoring of nitric oxide (NO) and oxygen (O2) in the striatum of immunocompromised NOD SCID mice. The latter strain has been utilized routinely in the establishment of humanized models of disease e.g., Parkinson’s disease. NOD SCID mice were implanted with highly sensitive and selective NO and O2 sensors that have been previously characterized both in vitro and in freely moving rats. Animals were systemically administered compounds that perturbed the amperometric current and confirmed sensor performance. Furthermore, the stability of the amperometric current was investigated and 24 h recordings examined. Saline injections caused transient changes in both currents that were not significant from baseline. l-NAME caused significant decreases in NO (p < 0.05) and O2 (p < 0.001) currents compared to saline. l-Arginine produced a significant increase (p < 0.001) in NO current, and chloral hydrate and Diamox (acetazolamide) caused significant increases in O2 signal (p < 0.01) compared against saline. The stability of both currents were confirmed over an eight-day period and analysis of 24-h recordings identified diurnal variations in both signals. These findings confirm the efficacy of the amperometric sensors to perform continuous and reliable recordings in immunocompromised mice. View Full-Text
Keywords: amperometry; nitric oxide; oxygen; real-time; characterization; NOD SCID; immunocompromised amperometry; nitric oxide; oxygen; real-time; characterization; NOD SCID; immunocompromised
Show Figures

Figure 1

MDPI and ACS Style

Reid, C.H.; Finnerty, N.J. Long Term Amperometric Recordings in the Brain Extracellular Fluid of Freely Moving Immunocompromised NOD SCID Mice. Sensors 2017, 17, 419.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop