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Sensors 2014, 14(4), 7026-7040; https://doi.org/10.3390/s140407026

Quorum Sensing Activity of Aeromonas Caviae Strain YL12, A Bacterium Isolated from Compost

Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
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Received: 4 March 2014 / Revised: 6 April 2014 / Accepted: 10 April 2014 / Published: 22 April 2014
(This article belongs to the Section Biosensors)
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Abstract

Quorum sensing is a well-studied cell-to-cell communication method that involves a cell-density dependent regulation of genes expression mediated by signalling molecules. In this study, a bacterium isolated from a plant material compost pile was found to possess quorum sensing activity based on bioassay screening. Isolate YL12 was identified using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry and molecular typing using rpoD gene which identified the isolate as Aeromonas caviae. High resolution tandem mass spectrometry was subsequently employed to identify the N-acyl homoserine lactone profile of Aeromonas caviae YL12 and confirmed that this isolate produced two short chain N-acyl homoserine lactones, namely C4-HSL and C6, and the production was observed to be cell density-dependent. Using the thin layer chromatography (TLC) bioassay, both AHLs were found to activate C. violaceum CV026, whereas only C6-HSL was revealed to induce bioluminescence expression of E. coli [pSB401]. The data presented in this study will be the leading steps in understanding the role of quorum sensing in Aeromonas caviae strain YL12. View Full-Text
Keywords: Cell-to-cell communication; C4-HSL (N-butyryl-L-homoserine lactone); C6-HSL (N-hexanoyl-L-homoserine lactone); N-acyl homoserine lactone (AHL); MALDI-TOF; triple quodruopole liquid chromatography mass spectrometry (LCMS/MS); Pacific Biosciences; RpoD; thin liquid chromatography (TLC) Cell-to-cell communication; C4-HSL (N-butyryl-L-homoserine lactone); C6-HSL (N-hexanoyl-L-homoserine lactone); N-acyl homoserine lactone (AHL); MALDI-TOF; triple quodruopole liquid chromatography mass spectrometry (LCMS/MS); Pacific Biosciences; RpoD; thin liquid chromatography (TLC)
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Lim, Y.-L.; Ee, R.; Yin, W.-F.; Chan, K.-G. Quorum Sensing Activity of Aeromonas Caviae Strain YL12, A Bacterium Isolated from Compost. Sensors 2014, 14, 7026-7040.

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