Placental Transcription Profiling in 6–23 Weeks’ Gestation Reveals Differential Transcript Usage in Early Development
Abstract
:1. Introduction
2. Results
2.1. Changes in the Placental Transcriptome from Early to Mid Gestation Are Highly Enriched for Genes Involved in Cell Migration and Transmembrane Signaling
2.2. Variable Transcript Expression Masks Differential Gene Expression
2.3. Global Differences in Transcript Usage Were Observed from Early to Mid Gestation
3. Discussion
4. Materials and Methods
4.1. Data Information and Ethics Statement
4.2. Data Processing
4.3. Differential Expression Analysis
4.4. Differential Transcript Usage
4.5. Gene Ontology Enrichment
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Category | Term | Ontology | * DE in Term | FDR |
---|---|---|---|---|
GO:0005886 | plasma MEMBRANE | CC | 637 (21.0%) | |
GO:0031226 | intrinsic component of plasma MEMBRANE | CC | 237 (28.4%) | |
GO:0005887 | integral component of plasma MEMBRANE | CC | 228 (28.9%) | 1.34 × 10−28 |
GO:0016021 | integral component of MEMBRANE | CC | 559 (19.9%) | |
GO:0004888 | transmembrane signaling receptor ACTIVITY | MF | 137 (31.6%) | |
GO:0005102 | signaling receptor BINDING | MF | 220 (24.7%) | |
GO:0016477 | cell MIGRATION | BP | 238 (23.8%) | 6.55 × 10−18 |
GO:0070887 | CELLULAR RESPONSE to chemical stimulus | BP | 414 (20.0%) | |
GO:0006952 | defense response | BP | 232 (23.7%) | 3.52 × 10−17 |
GO:0071310 | CELLULAR RESPONSE to organic substance | BP | 342 (20.7%) | 3.29 × 10−16 |
GO:0005539 | glycosaminoglycan BINDING | MF | 59 (44.7%) | |
GO:0030334 | REGULATION of cell MIGRATION | BP | 168 (25.8%) | |
GO:0048018 | receptor ligand ACTIVITY | MF | 68 (39.8%) | |
GO:0007166 | cell surface receptor signaling pathway | BP | 355 (20.1%) | |
GO:0051270 | REGULATION of cellular component movement | BP | 180 (24.8%) | |
GO:2000145 | REGULATION of cell motility | BP | 171 (25.1%) | |
GO:0030546 | signaling receptor activator ACTIVITY | MF | 69 (38.5%) | |
GO:0008201 | heparin BINDING | MF | 48 (49.0%) | |
GO:0006935 | chemotaxis | BP | 109 (29.9%) | |
GO:0031982 | vesicle | CC | 509 (18.1%) |
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Bogias, K.J.; Pederson, S.M.; Leemaqz, S.; Smith, M.D.; McAninch, D.; Jankovic-Karasoulos, T.; McCullough, D.; Wan, Q.; Bianco-Miotto, T.; Breen, J.; et al. Placental Transcription Profiling in 6–23 Weeks’ Gestation Reveals Differential Transcript Usage in Early Development. Int. J. Mol. Sci. 2022, 23, 4506. https://doi.org/10.3390/ijms23094506
Bogias KJ, Pederson SM, Leemaqz S, Smith MD, McAninch D, Jankovic-Karasoulos T, McCullough D, Wan Q, Bianco-Miotto T, Breen J, et al. Placental Transcription Profiling in 6–23 Weeks’ Gestation Reveals Differential Transcript Usage in Early Development. International Journal of Molecular Sciences. 2022; 23(9):4506. https://doi.org/10.3390/ijms23094506
Chicago/Turabian StyleBogias, Konstantinos J., Stephen M. Pederson, Shalem Leemaqz, Melanie D. Smith, Dale McAninch, Tanja Jankovic-Karasoulos, Dylan McCullough, Qianhui Wan, Tina Bianco-Miotto, James Breen, and et al. 2022. "Placental Transcription Profiling in 6–23 Weeks’ Gestation Reveals Differential Transcript Usage in Early Development" International Journal of Molecular Sciences 23, no. 9: 4506. https://doi.org/10.3390/ijms23094506