Benefits of Usage of Immobilized Silver Nanoparticles as Pseudomonas aeruginosa Antibiofilm Factors
Abstract
:1. Introduction
2. Results
2.1. Silver Nanoparticles Immobilized on SiO2 and TiO2
2.2. Antibiotic Susceptibility
2.3. Prevalence of silE Gene in Tested Bacterial Strains
2.4. Activity of SiO2/Ag0, TiO2/Ag0, SiO2, and TiO2 against Planktonic Forms (Minimal Inhibitory Concentration (MIC) Determination)
2.5. Activity of SiO2/Ag0 and TiO2/Ag0 against Biofilm Forms (Minimal Biofilm Inhibitory Concentration (MBIC) Determination)
2.6. Antibiofilm Activity of SiO2/Ag0 and TiO2/Ag0
2.7. The Viability of P. aeruginosa Clinical Strains in the Biofilm in the Presence of SiO2/Ag0 and TiO2/Ag0
2.8. Comparison of SiO2/Ag0 and TiO2/Ag0 Antibiofilm Activity against the Reference PAO1 Strain and Clinical Strains of P. aeruginosa
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains
4.2. Silver Nanoforms (SiO2/Ag0, TiO2/Ag0)
4.3. Antibiotic Susceptibility
4.4. Prevalence of Silver Resistance in Tested Bacteria Strains
4.5. Preparation of Bacterial Suspensions
4.6. Determination of the Minimal Inhibitory Concentration (MIC)
4.7. Determination of the Minimal Biofilm Inhibitory Concentration (MBIC)
4.8. Effects of SiO2/Ag0 and TiO2/Ag0 on Biofilm Formation
4.9. Effects of SiO2/Ag0 and TiO2/Ag0 on the Count of Live Bacteria in Biofilm
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample | Average Size of SiO2, TiO2 (nm) | Average Size of Ag0 (nm) | Surface Area (m2/g) | Pore Size (nm) | Content of Silver (% w/w) |
---|---|---|---|---|---|
SiO2/Ag0 | 123.73 (±25.86) | 17.91 (±3.37) | 332.0 ± 14.0 | 8.0 ± 2.0 | 10.0 |
TiO2/Ag0 | 154.33 (±15.98) | 16.57 (±5.01) | 424.0 ± 8.0 | 4.0 ± 2.0 | 10.0 |
Origin | Strain Number * | AN | CAZ | CIP | CL | IPM | MEM | TZP | MBL |
---|---|---|---|---|---|---|---|---|---|
broncho-alveolar lavage fluid | 0013 | S | R | R | S | R | R | R | - |
0024 | R | R | R | I | R | R | R | - | |
3 | R | R | R | S | R | R | R | + | |
472 | R | R | S | S | R | I | R | - | |
669 | R | S | R | S | R | R | R | - | |
urine | 124 | S | R | I | S | I | S | I | - |
137 | S | R | I | S | I | S | I | - | |
300 | R | I | R | S | R | I | R | - | |
328 | R | I | R | S | R | R | I | - | |
407 | S | I | I | S | I | I | I | - | |
ATCC collection | PAO1 (15692) | S | R | R | S | R | S | R | - |
Origin | Strain Number | MIC (µg/mL) | |||
---|---|---|---|---|---|
SiO2/Ag0 | TiO2/Ag0 | SiO2 | TiO2 | ||
PAO1 (ATCC 15692) | 256 | 256 | >8192 | >8192 | |
bronchoalveolar lavage fluid | 0013 | 32 | 32 | >8192 | >8192 |
0024 | 32 | 16 | |||
3 | 32 | 16 | |||
472 | 32 | 16 | |||
669 | 16 | 16 | |||
urine | 124 | 128 | 64 | >8192 | >8192 |
137 | 32 | 32 | |||
300 | 64 | 64 | |||
328 | 16 | 16 | |||
407 | 32 | 32 |
Origin | Strain Number | MBIC (µg/mL) | |
---|---|---|---|
SiO2/Ag0 | TiO2/Ag0 | ||
PAO1 (ATCC 15692) | 512 | 512 | |
bronchoalveolar lavage fluid | 0013 | 64 | 128 |
0024 | 128 | 512 | |
3 | 128 | 512 | |
472 | 64 | 128 | |
669 | 32 | 64 | |
urine | 124 | 256 | 128 |
137 | 64 | 128 | |
300 | 128 | 128 | |
328 | 64 | 64 | |
407 | 64 | 256 |
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Korzekwa, K.; Kędziora, A.; Stańczykiewicz, B.; Bugla-Płoskońska, G.; Wojnicz, D. Benefits of Usage of Immobilized Silver Nanoparticles as Pseudomonas aeruginosa Antibiofilm Factors. Int. J. Mol. Sci. 2022, 23, 284. https://doi.org/10.3390/ijms23010284
Korzekwa K, Kędziora A, Stańczykiewicz B, Bugla-Płoskońska G, Wojnicz D. Benefits of Usage of Immobilized Silver Nanoparticles as Pseudomonas aeruginosa Antibiofilm Factors. International Journal of Molecular Sciences. 2022; 23(1):284. https://doi.org/10.3390/ijms23010284
Chicago/Turabian StyleKorzekwa, Kamila, Anna Kędziora, Bartłomiej Stańczykiewicz, Gabriela Bugla-Płoskońska, and Dorota Wojnicz. 2022. "Benefits of Usage of Immobilized Silver Nanoparticles as Pseudomonas aeruginosa Antibiofilm Factors" International Journal of Molecular Sciences 23, no. 1: 284. https://doi.org/10.3390/ijms23010284