Next Article in Journal
Exercise–Linked Irisin: Consequences on Mental and Cardiovascular Health in Type 2 Diabetes
Previous Article in Journal
Neuroinflammation in Prion Disease
Previous Article in Special Issue
Novel Transcript Discovery Expands the Repertoire of Pathologically-Associated, Long Non-Coding RNAs in Vascular Smooth Muscle Cells
Open AccessArticle

A Comprehensive miRNome Analysis of Macrophages Isolated from db/db Mice and Selected miRNAs Involved in Metabolic Syndrome-Associated Cardiac Remodeling

1
Department of Histology and Embryology, Collegium Anatomicum, Medical University of Warsaw, 02-004 Warsaw, Poland
2
Postgraduate School of Molecular Medicine, Collegium Anatomicum, Medical University of Warsaw, 02-004 Warsaw, Poland
3
Student Scientific Group, Department of Histology and Embryology, Collegium Anatomicum, Medical University of Warsaw, 02-004 Warsaw, Poland
4
Department of History of Medicine, Medical University of Warsaw, 00-575 Warsaw, Poland
5
Department of Immunology, Faculty of Biology, University of Warsaw, 02-096 Warsaw, Poland
6
Molecular Biology Laboratory, Department of Medical Biology, Cardinal Stefan Wyszyński Institute of Cardiology, 04-628 Warsaw, Poland
7
Department of Clinical Anatomy, Collegium Anatomicum, Medical University of Warsaw, 02-004 Warsaw, Poland
8
Department of Pathology, Collegium Anatomicum, Medical University of Warsaw, 02-004 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Yvan Devaux
Int. J. Mol. Sci. 2021, 22(4), 2197; https://doi.org/10.3390/ijms22042197
Received: 15 January 2021 / Revised: 19 February 2021 / Accepted: 20 February 2021 / Published: 23 February 2021
(This article belongs to the Special Issue RNAs in Brain and Heart Diseases - EU-CardioRNA COST Action)
Cardiac macrophages are known from various activities, therefore we presume that microRNAs (miRNAs) produced or released by macrophages in cardiac tissue have impact on myocardial remodeling in individuals with metabolic syndrome (MetS). We aim to assess the cardiac macrophage miRNA profile by selecting those miRNA molecules that potentially exhibit regulatory functions in MetS-related cardiac remodeling. Cardiac tissue macrophages from control and db/db mice (an animal model of MetS) were counted and sorted with flow cytometry, which yielded two populations: CD45+CD11b+CD64+Ly6Chi and CD45+CD11b+CD64+Ly6Clow. Total RNA was then isolated, and miRNA expression profiles were evaluated with Next Generation Sequencing. We successfully sequenced 1400 miRNAs in both macrophage populations: CD45+CD11b+CD64+Ly6Chi and CD45+CD11b+CD64+Ly6Clow. Among the 1400 miRNAs, about 150 showed different expression levels in control and db/db mice and between these two subpopulations. At least 15 miRNAs are possibly associated with MetS pathology in cardiac tissue due to direct or indirect regulation of the expression of miRNAs for proteins involved in angiogenesis, fibrosis, or inflammation. In this paper, for the first time we describe the miRNA transcription profile in two distinct macrophage populations in MetS-affected cardiac tissue. Although the results are preliminary, the presented data provide a foundation for further studies on intercellular cross-talk/molecular mechanism(s) involved in the regulation of MetS-related cardiac remodeling. View Full-Text
Keywords: cardiac macrophages; miRNA; metabolic syndrome; myocardial remodeling cardiac macrophages; miRNA; metabolic syndrome; myocardial remodeling
Show Figures

Figure 1

MDPI and ACS Style

Niderla-Bielińska, J.; Ścieżyńska, A.; Moskalik, A.; Jankowska-Steifer, E.; Bartkowiak, K.; Bartkowiak, M.; Kiernozek, E.; Podgórska, A.; Ciszek, B.; Majchrzak, B.; Ratajska, A. A Comprehensive miRNome Analysis of Macrophages Isolated from db/db Mice and Selected miRNAs Involved in Metabolic Syndrome-Associated Cardiac Remodeling. Int. J. Mol. Sci. 2021, 22, 2197. https://doi.org/10.3390/ijms22042197

AMA Style

Niderla-Bielińska J, Ścieżyńska A, Moskalik A, Jankowska-Steifer E, Bartkowiak K, Bartkowiak M, Kiernozek E, Podgórska A, Ciszek B, Majchrzak B, Ratajska A. A Comprehensive miRNome Analysis of Macrophages Isolated from db/db Mice and Selected miRNAs Involved in Metabolic Syndrome-Associated Cardiac Remodeling. International Journal of Molecular Sciences. 2021; 22(4):2197. https://doi.org/10.3390/ijms22042197

Chicago/Turabian Style

Niderla-Bielińska, Justyna; Ścieżyńska, Aneta; Moskalik, Aneta; Jankowska-Steifer, Ewa; Bartkowiak, Krzysztof; Bartkowiak, Mateusz; Kiernozek, Ewelina; Podgórska, Anna; Ciszek, Bogdan; Majchrzak, Barbara; Ratajska, Anna. 2021. "A Comprehensive miRNome Analysis of Macrophages Isolated from db/db Mice and Selected miRNAs Involved in Metabolic Syndrome-Associated Cardiac Remodeling" Int. J. Mol. Sci. 22, no. 4: 2197. https://doi.org/10.3390/ijms22042197

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop