Genome-Wide Analysis of Smad7-Mediated Transcription in Mouse Embryonic Stem Cells
Abstract
:1. Introduction
2. Results
2.1. Nuclear Smad7 Regulates Genes Involved in ESC Self-Renewal and Pluripotency Maintenance
2.2. Genome-Wide Identification and Characterization of Smad7 Binding Sites in ESCs
2.3. Smad7 Binds to Promoters of Genes Involved in ESC Self-Renewal
2.4. Smad7 Participates to ESC Regulatory Network
3. Discussion
4. Materials and Methods
4.1. Embryonic Stem Cell Culture
4.2. DNA Construct and shRNA
4.3. Transient Transfection
4.4. In Vivo Biotinylation
4.5. Subcellular Fractionation
4.6. Protein Extraction and Western Blotting
4.7. Immunofluorescence
4.8. AP Staining
4.9. RNA Extraction and RT-qPCR
4.10. RNA-seq Library Preparation
4.11. RNA-seq Analysis
4.12. Bio-ChIP-seq
4.13. Bio-ChIP-seq Library Preparation
4.14. Bio-ChIP-seq Data Analysis
4.15. Bio-ChIP Assay
4.16. Published Datasets
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Meng, G.; Lauria, A.; Maldotti, M.; Anselmi, F.; Polignano, I.L.; Rapelli, S.; Donna, D.; Oliviero, S. Genome-Wide Analysis of Smad7-Mediated Transcription in Mouse Embryonic Stem Cells. Int. J. Mol. Sci. 2021, 22, 13598. https://doi.org/10.3390/ijms222413598
Meng G, Lauria A, Maldotti M, Anselmi F, Polignano IL, Rapelli S, Donna D, Oliviero S. Genome-Wide Analysis of Smad7-Mediated Transcription in Mouse Embryonic Stem Cells. International Journal of Molecular Sciences. 2021; 22(24):13598. https://doi.org/10.3390/ijms222413598
Chicago/Turabian StyleMeng, Guohua, Andrea Lauria, Mara Maldotti, Francesca Anselmi, Isabelle Laurence Polignano, Stefania Rapelli, Daniela Donna, and Salvatore Oliviero. 2021. "Genome-Wide Analysis of Smad7-Mediated Transcription in Mouse Embryonic Stem Cells" International Journal of Molecular Sciences 22, no. 24: 13598. https://doi.org/10.3390/ijms222413598