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Open AccessArticle

Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study

1
Department of Medical and Surgical Sciences, Institute of Neurology, University “Magna Graecia”, Germaneto, 88100 Catanzaro, Italy
2
Institute of Molecular Bioimaging and Physiology (IBFM), National Research Council (CNR), Via F.Cervi 93, 20090 Segrate-Milan, Italy
3
Department of Experimental and Clinical Medicine, University “Magna Graecia” of Catanzaro, 88100 Catanzaro, Italy
4
Neuroscience Research Center, University “Magna Graecia” of Catanzaro, 88100 Catanzaro, Italy
5
Department of Physics “Giuseppe Occhialini”, University of Milan-Bicocca, Piazza della Scienza 3, 20126 Milan, Italy
6
Institute of Molecular Bioimaging and Physiology (IBFM), National Research Council (CNR), Section of Germaneto, 88100 Catanzaro, Italy
*
Authors to whom correspondence should be addressed.
These authors contributed equally.
Int. J. Mol. Sci. 2021, 22(2), 702; https://doi.org/10.3390/ijms22020702
Received: 22 December 2020 / Revised: 6 January 2021 / Accepted: 10 January 2021 / Published: 12 January 2021
(This article belongs to the Special Issue ncRNAS in Therapeutics)
MicroRNAs (miRNAs) are small noncoding RNAs that have emerged as new potential epigenetic biomarkers. Here, we evaluate the efficacy of six circulating miRNA previously described in the literature as biomarkers for the diagnosis of temporal lobe epilepsy (TLE) and/or as predictive biomarkers to antiepileptic drug response. We measured the differences in serum miRNA levels by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assays in a cohort of 27 patients (14 women and 13 men; mean ± SD age: 43.65 ± 17.07) with TLE compared to 20 healthy controls (HC) matched for sex, age and ethnicity (11 women and 9 men; mean ± SD age: 47.5 ± 9.1). Additionally, patients were classified according to whether they had drug-responsive (n = 17) or drug-resistant (n = 10) TLE. We have investigated any correlations between miRNAs and several electroclinical parameters. Three miRNAs (miR-142, miR-146a, miR-223) were significantly upregulated in patients (expressed as average expression ± SD). In detail, miR-142 expression was 0.40 ± 0.29 vs. 0.16 ± 0.10 in TLE patients compared to HC (t-test, p < 0.01), miR-146a expression was 0.15 ± 0.11 vs. 0.07 ± 0.04 (t-test, p < 0.05), and miR-223 expression was 6.21 ± 3.65 vs. 1.23 ± 0.84 (t-test, p < 0.001). Moreover, results obtained from a logistic regression model showed the good performance of miR-142 and miR-223 in distinguishing drug-sensitive vs. drug-resistant TLE. The results of this pilot study give evidence that miRNAs are suitable targets in TLE and offer the rationale for further confirmation studies in larger epilepsy cohorts. View Full-Text
Keywords: temporal lobe epilepsy; miRNAs; diagnosis; prognosis; antiseizure medications; ASMs temporal lobe epilepsy; miRNAs; diagnosis; prognosis; antiseizure medications; ASMs
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MDPI and ACS Style

De Benedittis, S.; Fortunato, F.; Cava, C.; Gallivanone, F.; Iaccino, E.; Caligiuri, M.E.; Castiglioni, I.; Bertoli, G.; Manna, I.; Labate, A.; Gambardella, A. Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study. Int. J. Mol. Sci. 2021, 22, 702.

AMA Style

De Benedittis S, Fortunato F, Cava C, Gallivanone F, Iaccino E, Caligiuri ME, Castiglioni I, Bertoli G, Manna I, Labate A, Gambardella A. Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study. International Journal of Molecular Sciences. 2021; 22(2):702.

Chicago/Turabian Style

De Benedittis, Selene; Fortunato, Francesco; Cava, Claudia; Gallivanone, Francesca; Iaccino, Enrico; Caligiuri, Maria E.; Castiglioni, Isabella; Bertoli, Gloria; Manna, Ida; Labate, Angelo; Gambardella, Antonio. 2021. "Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study" Int. J. Mol. Sci. 22, no. 2: 702.

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