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Article

Directed Recovery and Molecular Characterization of Antibiotic Resistance Plasmids from Cheese Bacteria

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Departamento de Microbiología y Bioquímica, Instituto de Productos Lácteos de Asturias (IPLA), Consejo Superior de Investigaciones Científicas (CSIC), Paseo Río Linares s/n, 33300 Villaviciosa, Spain
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Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Avenida de Roma s/n, 33011 Oviedo, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Carlos Juan Nicolau
Int. J. Mol. Sci. 2021, 22(15), 7801; https://doi.org/10.3390/ijms22157801
Received: 10 June 2021 / Revised: 13 July 2021 / Accepted: 19 July 2021 / Published: 21 July 2021
(This article belongs to the Special Issue Antibiotic Resistance: Appearance, Evolution, and Spread)
Resistance to antimicrobials is a growing problem of worldwide concern. Plasmids are thought to be major drivers of antibiotic resistance spread. The present work reports a simple way to recover replicative plasmids conferring antibiotic resistance from the bacteria in cheese. Purified plasmid DNA from colonies grown in the presence of tetracycline and erythromycin was introduced into plasmid-free strains of Lactococcus lactis, Lactiplantibacillus plantarum and Lacticaseibacillus casei. Following antibiotic selection, the plasmids from resistant transformants were isolated, analyzed by restriction enzyme digestion, and sequenced. Seven patterns were obtained for the tetracycline-resistant colonies, five from L. lactis, and one each from the lactobacilli strains, as well as a single digestion profile for the erythromycin-resistant transformants obtained in L. lactis. Sequence analysis respectively identified tet(S) and ermB in the tetracycline- and erythromycin-resistance plasmids from L. lactis. No dedicated resistance genes were detected in plasmids conferring tetracycline resistance to L. casei and L. plantarum. The present results highlight the usefulness of the proposed methodology for isolating functional plasmids that confer antibiotic resistance to LAB species, widen our knowledge of antibiotic resistance in the bacteria that inhabit cheese, and emphasize the leading role of plasmids in the spread of resistance genes via the food chain. View Full-Text
Keywords: antibiotic resistance; horizontal gene transfer; plasmids; tetracycline and erythromycin resistance; artisanal cheeses; lactic acid bacteria antibiotic resistance; horizontal gene transfer; plasmids; tetracycline and erythromycin resistance; artisanal cheeses; lactic acid bacteria
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MDPI and ACS Style

Flórez, A.B.; Vázquez, L.; Rodríguez, J.; Mayo, B. Directed Recovery and Molecular Characterization of Antibiotic Resistance Plasmids from Cheese Bacteria. Int. J. Mol. Sci. 2021, 22, 7801. https://doi.org/10.3390/ijms22157801

AMA Style

Flórez AB, Vázquez L, Rodríguez J, Mayo B. Directed Recovery and Molecular Characterization of Antibiotic Resistance Plasmids from Cheese Bacteria. International Journal of Molecular Sciences. 2021; 22(15):7801. https://doi.org/10.3390/ijms22157801

Chicago/Turabian Style

Flórez, Ana B., Lucía Vázquez, Javier Rodríguez, and Baltasar Mayo. 2021. "Directed Recovery and Molecular Characterization of Antibiotic Resistance Plasmids from Cheese Bacteria" International Journal of Molecular Sciences 22, no. 15: 7801. https://doi.org/10.3390/ijms22157801

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