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Review

How Single-Molecule Localization Microscopy Expanded Our Mechanistic Understanding of RNA Polymerase II Transcription

1
Department of Biology of the Cell Nucleus, Institute of Molecular Genetics of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic
2
Faculty of Science, Charles University, Albertov 6, 128 00 Prague, Czech Republic
3
Microscopy Centre, Institute of Molecular Genetics of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Kiryl Piatkevich
Int. J. Mol. Sci. 2021, 22(13), 6694; https://doi.org/10.3390/ijms22136694
Received: 7 June 2021 / Revised: 17 June 2021 / Accepted: 19 June 2021 / Published: 22 June 2021
Classical models of gene expression were built using genetics and biochemistry. Although these approaches are powerful, they have very limited consideration of the spatial and temporal organization of gene expression. Although the spatial organization and dynamics of RNA polymerase II (RNAPII) transcription machinery have fundamental functional consequences for gene expression, its detailed studies have been abrogated by the limits of classical light microscopy for a long time. The advent of super-resolution microscopy (SRM) techniques allowed for the visualization of the RNAPII transcription machinery with nanometer resolution and millisecond precision. In this review, we summarize the recent methodological advances in SRM, focus on its application for studies of the nanoscale organization in space and time of RNAPII transcription, and discuss its consequences for the mechanistic understanding of gene expression. View Full-Text
Keywords: cell nucleus; gene expression; transcription foci; transcription factors; super-resolution microscopy; structured illumination; stimulated emission depletion; stochastic optical reconstruction; photoactivation cell nucleus; gene expression; transcription foci; transcription factors; super-resolution microscopy; structured illumination; stimulated emission depletion; stochastic optical reconstruction; photoactivation
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MDPI and ACS Style

Hoboth, P.; Šebesta, O.; Hozák, P. How Single-Molecule Localization Microscopy Expanded Our Mechanistic Understanding of RNA Polymerase II Transcription. Int. J. Mol. Sci. 2021, 22, 6694. https://doi.org/10.3390/ijms22136694

AMA Style

Hoboth P, Šebesta O, Hozák P. How Single-Molecule Localization Microscopy Expanded Our Mechanistic Understanding of RNA Polymerase II Transcription. International Journal of Molecular Sciences. 2021; 22(13):6694. https://doi.org/10.3390/ijms22136694

Chicago/Turabian Style

Hoboth, Peter, Ondřej Šebesta, and Pavel Hozák. 2021. "How Single-Molecule Localization Microscopy Expanded Our Mechanistic Understanding of RNA Polymerase II Transcription" International Journal of Molecular Sciences 22, no. 13: 6694. https://doi.org/10.3390/ijms22136694

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